Cytotoxic Effects of Verbascoside on MCF-7 and MDA-MB-231

Turkish Journal of Pharmaceutical Sciences

article

Hülya ŞENOL¹  Pinar TULAY²  Mahmut Cerkez ERGÖREN²  Azmi HANOĞLU⁴  İhsan ÇALIŞ⁴  Gamze MOCAN⁵ 

[1] Near East University Faculty of Medicine, Department of Medical Biology;Near East University Faculty of Medicine, Department of Medical Genetics;Near East University, Desam Research Institute;Near East University Faculty of Pharmacy, Department of Pharmacognosy;Near East University Faculty of Medicine, Department of Medical Pathology

关键词: Cytotoxicity;    MCF-7;    MDA-MB-231;    Phlomis nissolii L.;    verbascoside;   

DOI  :  10.4274/tjps.galenos.2021.36599

来源: Galenos Yayinevi

【 摘 要 】

Objectives: Verbascoside, also known as acteoside/kusaginin, has attracted a great attention due to its pharmacological features. In this study, we aimed to determine the cytotoxic effects of pure verbascoside isolated from Phlomis nissolii L. plant in both MCF-7 and MDA-MB-231 cell lines in vitro. Materials and Methods: MCF-7 and MDA-MB 231 cells were treated with verbascoside (100, 48, 25, 10, 1, 0.5, and 0.1 μM) for 24, 48, and 72 hours. Cytotoxic effect of verbascoside in MCF-7 and MDA-MB-231 cells was assessed using TEBU-BIO cell counting kit 8. Results and Conclusion: IC50 values for 24, 48, and 72 h verbascoside exposure of MCF-7 cells were determined as 0.127, 0.2174, and 0.2828 μM, respectively. R2 values were calculated as 0.9630, 0.8789 and 0.8752, respectively. Two-Way ANOVA multiple comparison test results showed that 100 μM verbascoside has the highest cytotoxic effect on MCF-7 breast cancer (BC) cells after 72 h of exposure. IC50 values for 24, 48 and 72 h verbascoside exposure of MDA-MB 231 cells were determined as 0.1597, 0.2584 and 0.2563 μM, respectively and R2 values were calculated as 0.8438, 0.5107 and 0.9203, respectively. Two-Way ANOVA multiple comparisons test results showed that 100 μM verbascoside has the highest cytotoxic effect on MDA-MB 231 BC cells after 24, 48 and 72 h of exposure.

【 授权许可】

Unknown