T-cell responses to SARS-CoV-2 in unexposed South African women

Gates Open Research

article

Marta C. Nunes¹  Michael J. Johnson³  Gaurav Kwatra¹  Adriana Weinberg³  Shabir A. Madhi¹ 

[1] South African Medical Research Council, Vaccines and Infectious Diseases Analytics Research Unit, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand;Department of Science and Technology/National Research Foundation, South African Research Chair Initiative in Vaccine Preventable Diseases, Faculty of Health Sciences, University of the Witwatersrand;Department of Pediatrics, Medicine and Pathology, University of Colorado, Anschutz Medical Campus

关键词: cell mediated immunity;    SARS-CoV-2;    interferon gamma;    interleukin 2;   

DOI  :  10.12688/gatesopenres.13373.2

学科分类：电子与电气工程

来源: American Journal Of Pharmtech Research

【 摘 要 】

Background: A potential explanation for the fact that the high rate of infection of SARS-CoV-2 in South Africa did not translate into high rates of severe illness and death may be the presence of cross-reactive immunity induced by common cold coronaviruses (CCoV). Methods: We used SARS-CoV-2 peptide pools and whole virus antigen to stimulate peripheral blood mononuclear cells collected pre-2020 from South African women. Dual-colour FluoroSpot assay was used to measure interferon gamma (IFNγ) and interleukin 2 (IL2) production. Results: Among the 97 study participants, IFNγ responses were observed in 29.9% of the women and IL2 among 39.2%. Overall, 51.6% of women demonstrated response to at least one stimulant. Conclusion: We demonstrate the presence of cross-reactive immunity to SARS-CoV-2, which might have been induced by past exposure to CCoV.

【 授权许可】

CC BY