Sheep condyle model evaluation of bone marrow cell concentrate combined with a scaffold for repair of large osteochondral defects

Bone & Joint Research

article

Maryam Tamaddon¹  Gordon Blunn²  Wei Xu³  Maria Elena Alemán Domínguez⁴  Mario Monzón⁴  James Donaldson⁵  John Skinner¹  Timothy R. Arnett⁶  Ling Wang⁷  Chaozong Liu¹ 

[1] Institute of Orthopaedic & Musculoskeletal Science, Division of Surgery & Interventional Science, University College London, Royal National Orthopaedic Hospital;School of Pharmacy and Biomedical Sciences, University of Portsmouth;Beijing Advanced Innovation Center for Materials Genome Engineering, Institute for Advanced Materials and Technology, State Key Laboratory for Advanced Metals and Materials, University of Science and Technology Beijing;Departamento de Ingeniería Mecánica, Universidad de Las Palmas de Gran Canaria;Knee and Hip Unit, Royal National Orthopaedic Hospital;Department of Cell and Developmental Biology, University College London;State Key Laboratory for Manufacturing System Engineering, School of Mechanical Engineering, Xi'an Jiaotong University

关键词: Bone marrow concentrate;    Osteochondral scaffold;    Minimally manipulated cells;    osteochondral defects;    Sheep;    condyles;    cartilage;    Bone marrow;    collagens;    force-plate;    tissue regeneration;    alcian blue;    sulphated glycosaminoglycans;   

DOI  :  10.1302/2046-3758.1010.BJR-2020-0504.R1

学科分类：骨科学

来源: British Editorial Society Of Bone And Joint Surgery

【 摘 要 】

AimsMinimally manipulated cells, such as autologous bone marrow concentrates (BMC), have been investigated in orthopaedics as both a primary therapeutic and augmentation to existing restoration procedures. However, the efficacy of BMC in combination with tissue engineering is still unclear. In this study, we aimed to determine whether the addition of BMC to an osteochondral scaffold is safe and can improve the repair of large osteochondral defects when compared to the scaffold alone.MethodsThe ovine femoral condyle model was used. Bone marrow was aspirated, concentrated, and used intraoperatively with a collagen/hydroxyapatite scaffold to fill the osteochondral defects (n = 6). Tissue regeneration was then assessed versus the scaffold-only group (n = 6). Histological staining of cartilage with alcian blue and safranin-O, changes in chondrogenic gene expression, microCT, peripheral quantitative CT (pQCT), and force-plate gait analyses were performed. Lymph nodes and blood were analyzed for safety.ResultsThe results six months postoperatively showed that there were no significant differences in bone regrowth and mineral density between BMC-treated animals and controls. A significant upregulation of messenger RNA (mRNA) for types I and II collagens in the BMC group was observed, but there were no differences in the formation of hyaline-like cartilage between the groups. A trend towards reduced sulphated glycosaminoglycans (sGAG) breakdown was detected in the BMC group but this was not statistically significant. Functional weightbearing was not affected by the inclusion of BMC.ConclusionOur results indicated that the addition of BMC to scaffold is safe and has some potentially beneficial effects on osteochondral-tissue regeneration, but not on the functional endpoint of orthopaedic interest.

【 授权许可】

CC BY-NC