Osteoblast function in patients with idiopathic osteonecrosis of the femoral head: implications for a possible novel therapy

Bone & Joint Research

article

Leila Maestro-Paramio¹  Eduardo García-Rey²  Fátima Bensiamar¹  Laura Saldaña¹ 

[1] Hospital Universitario La Paz-IdiPAZ;Departamento de Cirugía Ortopédica y Traumatología, Hospital Universitario La Paz-IdiPAZ

关键词: Osteonecrosis of the femoral head;    Idiopathic;    Osteoblasts;    osteoblasts;    Osteonecrosis of the femoral head (ONFH);    femoral head;    osteoarthritis (OA);    secretion;    mRNA;    RNA;    interleukin-6;    Alkaline phosphatase;    Trabecular bone;   

DOI  :  10.1302/2046-3758.109.BJR-2021-0016.R1

学科分类：骨科学

来源: British Editorial Society Of Bone And Joint Surgery

【 摘 要 】

AimsTo investigate whether idiopathic osteonecrosis of the femoral head (ONFH) is related to impaired osteoblast activities.MethodsWe cultured osteoblasts isolated from trabecular bone explants taken from the femoral head and the intertrochanteric region of patients with idiopathic ONFH, or from the intertrochanteric region of patients with osteoarthritis (OA), and compared their viability, mineralization capacity, and secretion of paracrine factors.ResultsOsteoblasts from the intertrochanteric region of patients with ONFH showed lower alkaline phosphatase (ALP) activity and mineralization capacity than osteoblasts from the same skeletal site in age-matched patients with OA, as well as lower messenger RNA (mRNA) levels of genes encoding osteocalcin and bone sialoprotein and higher osteopontin expression. In addition, osteoblasts from patients with ONFH secreted lower osteoprotegerin (OPG) levels than those from patients with OA, resulting in a higher receptor activator of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) ligand (RANKL)-to-OPG ratio. In patients with ONFH, osteoblasts from the femoral head showed reduced viability and mineralized nodule formation compared with osteoblasts from the intertrochanteric region. Notably, the secretion of the pro-resorptive factors interleukin-6 and prostaglandin E2 as well as the RANKL-to-OPG ratio were markedly higher in osteoblast cultures from the femoral head than in those from the intertrochanteric region.ConclusionIdiopathic ONFH is associated with a reduced mineralization capacity of osteoblasts and increased secretion of pro-resorptive factors.

【 授权许可】

CC BY-NC