期刊论文详细信息
Bone & Joint Research | |
The effect of TNF-α on osteoblasts in metal wear-induced periprosthetic bone loss | |
article | |
Rita Hameister1  Christoph H. Lohmann2  S. Thameem Dheen1  Gurpal Singh3  Charanjit Kaur1  | |
[1]Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore | |
[2]Department of Orthopaedic Surgery, Otto von Guericke University Magdeburg | |
[3]Centre for Orthopaedics | |
关键词: Bone loss; Endoplasmic reticulum stress; Implant loosening; Osteoblast; Tumour necrosis factor-alpha; | |
DOI : 10.1302/2046-3758.911.BJR-2020-0001.R2 | |
学科分类:骨科学 | |
来源: British Editorial Society Of Bone And Joint Surgery | |
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【 摘 要 】
AimsThis study aimed to examine the effects of tumour necrosis factor-alpha (TNF-α) on osteoblasts in metal wear-induced bone loss.MethodsTNF-α immunoexpression was examined in periprosthetic tissues of patients with failed metal-on-metal hip arthroplasties and also in myeloid MM6 cells after treatment with cobalt ions. Viability and function of human osteoblast-like SaOs-2 cells treated with recombinant TNF-α were studied by immunofluorescence, terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) assay, western blotting, and enzyme-linked immunosorbent assay (ELISA).ResultsMacrophages, lymphocytes, and endothelial cells displayed strong TNF-α immunoexpression in periprosthetic tissues containing metal wear debris. Colocalization of TNF-α with the macrophage marker CD68 and the pan-T cell marker CD3 confirmed TNF-α expression in these cells. Cobalt-treated MM6 cells secreted more TNF-α than control cells, reflecting the role of metal wear products in activating the TNF-α pathway in the myeloid cells. While TNF-α did not alter the immunoexpression of the TNF-receptor 1 (TNF-R1) in SaOs-2 cells, it increased the release of the soluble TNF-receptor 1 (sTNF-R1). There was also evidence for TNF-α-induced apoptosis. TNF-α further elicited the expression of the endoplasmic reticulum stress markers inositol-requiring enzyme (IRE)-1α, binding-immunoglobulin protein (BiP), and endoplasmic oxidoreductin1 (Ero1)-Lα. In addition, TNF-α decreased pro-collagen I α 1 secretion without diminishing its synthesis. TNF-α also induced an inflammatory response in SaOs-2 cells, as evidenced by the release of reactive oxygen and nitrogen species and the proinflammatory cytokine vascular endothelial growth factor.ConclusionThe results suggest a novel osteoblastic mechanism, which could be mediated by TNF-α and may be involved in metal wear debris-induced periprosthetic bone loss.【 授权许可】
CC BY-NC
【 预 览 】
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