Different composition of leucocytes in cortical and cancellous bone healing in a mouse model

Bone & Joint Research

article

L. Tätting¹  O. Sandberg²  M. Bernhardsson¹  J. Ernerudh³  P. Aspenberg†⁴ 

[1] Department of Clinical and Experimental Medicine, Orthopaedics, Linköping University;Principal Research Engineer, Department of Clinical and Experimental Medicine, Orthopaedics, Linköping University;Department of Clinical and Experimental Medicine and Department of Clinical Immunology and Transfusion Medicine, Linköping University;Linköping

关键词: Metaphyseal;    Diaphyseal;    Cortical;    Cancellous;    Intramembranous;    Fracture;   

DOI  :  10.1302/2046-3758.712.BJR-2017-0366.R2

学科分类：骨科学

来源: British Editorial Society Of Bone And Joint Surgery

【 摘 要 】

ObjectivesCortical and cancellous bone healing processes appear to be histologically different. They also respond differently to anti-inflammatory agents. We investigated whether the leucocyte composition on days 3 and 5 after cortical and cancellous injuries to bone was different, and compared changes over time using day 3 as the baseline.MethodsTen-week-old male C56/Bl6J mice were randomized to either cancellous injury in the proximal tibia or cortical injury in the femoral diaphysis. Regenerating tissues were analyzed with flow cytometry at days 3 and 5, using panels with 15 antibodies for common macrophage and lymphocyte markers. The cellular response from day 3 to 5 was compared in order to identify differences in how cancellous and cortical bone healing develop.ResultsBetween day 3 and 5, the granulocytes increased in the cancellous model, whereas the lymphocytes (T cells, B cells, NK cells) and monocytes (CD11b+, F4/80+, CD206+, CD14+) increased in the cortical model.ConclusionThese results suggest an acute type of inflammation in cancellous bone healing, and a more chronic inflammation in cortical healing. This might explain, in part, why cancellous healing is faster and more resistant to anti-inflammatory drugs than are diaphyseal fractures.

【 授权许可】

CC BY-NC