The biological response to laser-aided direct metal-coated Titanium alloy (Ti6Al4V)

Bone & Joint Research

article

T. Shin¹  D. Lim¹  Y. S. Kim³  S. C. Kim³  W. L. Jo³  Y. W. Lim³ 

[1] Department of Mechanical Engineering, Sejong University;Corentec, Central R&D Center;Department of Orthopaedic Surgery, Seoul St. Mary’s Hospital, School of Medicine, The Catholic University of Korea

关键词: Coating technology;    Metal 3D printing;    Porous coating;   

DOI  :  10.1302/2046-3758.75.BJR-2017-0222.R1

学科分类：骨科学

来源: British Editorial Society Of Bone And Joint Surgery

【 摘 要 】

ObjectivesLaser-engineered net shaping (LENS) of coated surfaces can overcome the limitations of conventional coating technologies. We compared the in vitro biological response with a titanium plasma spray (TPS)-coated titanium alloy (Ti6Al4V) surface with that of a Ti6Al4V surface coated with titanium using direct metal fabrication (DMF) with 3D printing technologies.MethodsThe in vitro ability of human osteoblasts to adhere to TPS-coated Ti6Al4V was compared with DMF-coating. Scanning electron microscopy (SEM) was used to assess the structure and morphology of the surfaces. Biological and morphological responses to human osteoblast cell lines were then examined by measuring cell proliferation, alkaline phosphatase activity, actin filaments, and RUNX2 gene expression.ResultsMorphological assessment of the cells after six hours of incubation using SEM showed that the TPS- and DMF-coated surfaces were largely covered with lamellipodia from the osteoblasts. Cell adhesion appeared similar in both groups. The differences in the rates of cell proliferation and alkaline phosphatase activities were not statistically significant.ConclusionsThe DMF coating applied using metal 3D printing is similar to the TPS coating, which is the most common coating process used for bone ingrowth. The DMF method provided an acceptable surface structure and a viable biological surface. Moreover, this method is automatable and less complex than plasma spraying.

【 授权许可】

CC BY-NC