Probable impact of age and hypoxia on proliferation and microRNA expression profile of bone marrow-derived human mesenchymal stem cells

PeerJ

article

Norlaily Mohd Ali¹  Lily Boo¹  Swee Keong Yeap²  Huynh Ky³  Dilan A. Satharasinghe²  Woan Charn Liew²  Han Kiat Ong¹  Soon Keng Cheong¹  Tunku Kamarul⁷ 

[1] Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman;Institute of Bioscience, Universiti Putra Malaysia;Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia;Department of Agriculture Genetics and Breeding, College of Agriculture and Applied Biology, Cantho University;Faculty of Veterinary Medicine and Animal Science, University of Peradeniya;Cryocord Sdn Bhd;Tissue Engineering Group ,(TEG), National Orthopaedic Center of Excellence for Research and Learning ,(NOCERAL), Faculty of Medicine, Universiti Malaya

关键词: Hypoxia;    Differentiation;    Age;    Proliferation;    Bone-marrow;    Mesenchymal stem cells;    Next generation sequencing;    MicroRNA;    Differentiation;   

DOI  :  10.7717/peerj.1536

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Decline in the therapeutic potential of bone marrow-derived mesenchymal stem cells (MSC) is often seen with older donors as compared to young. Although hypoxia is known as an approach to improve the therapeutic potential of MSC in term of cell proliferation and differentiation capacity, its effects on MSC from aged donors have not been well studied. To evaluate the influence of hypoxia on different age groups, MSC from young (60 years) donors were expanded under hypoxic (5% O2) and normal (20% O2 old aged (normoxia).

【 授权许可】

CC BY