Isolation and characterization of eight polymorphic microsatellites for the spotted spiny lobster, Panulirus guttatus

PeerJ

article

Nathan Truelove¹  Donald C. Behringer³  Mark J. Butler IV⁴  Richard F. Preziosi¹ 

[1] Faculty of Life Sciences, University of Manchester;Smithsonian Museum of Natural History, Smithsonian Marine Station;School of Forest Resources and Conservation & Emerging Pathogens Institute, University of Florida;Department of Biological Sciences, Old Dominion University

关键词: Conservation;    Population genetics;    Neutral marker;    Connectivity;   

DOI  :  10.7717/peerj.1467

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Microsatellite sequences were isolated from enriched genomic libraries of the spotted spiny lobster, Panulirus guttatus using 454 pyrosequencing. Twenty-nine previously developed polymerase chain reaction primer pairs of Panulirus argus microsatellite loci were also tested for cross-species amplification in Panulirus guttatus. In total, eight consistently amplifying, and polymorphic loci were characterized for 57 individuals collected in the Florida Keys and Bermuda. The number of alleles per locus ranged from 8 to 20 and observed heterozygosities ranged from 0.409 to 0.958. Significant deviations from Hardy–Weinberg equilibrium were found in one locus from Florida and three loci from Bermuda. Quality control testing indicated that all loci were easy to score, highly polymorphic and showed no evidence of linkage disequilibrium. Null alleles were detected in three loci with moderate frequencies ranging from (20% to 22%). These eight microsatellites provide novel molecular markers for future conservation genetics research of P. guttatus.

【 授权许可】

CC BY