De novo assembly and characterization of the Chinese three-keeled pond turtle ( Mauremys reevesii ) transcriptome: presence of longevity-related genes

PeerJ

article

Huazong Yin¹  Liuwang Nie¹  Feifei Zhao¹  Huaxing Zhou¹  Haifeng Li¹  Xianmei Dong¹  Huanhuan Zhang¹  Yuqin Wang¹  Qiong Shi¹  Jun Li¹ 

[1]College of Life Science, Anhui Normal University, Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui

关键词: Mauremys reevesii;    de novo assembly;    RNA-seq;    Telomere;    Telomerase activity;    Shelterin complex;    Longevity;   

DOI  :  10.7717/peerj.2062

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Mauremys reevesii (Geoemydidae) is one of the most common and widespread semi-aquatic turtles in East Asia. The unusually long lifespan of some individuals makes this turtle species a potentially useful model organism for studying the molecular basis of longevity. In this study, pooled total RNA extracted from liver, spleen and skeletal-muscle of three adult individuals were sequenced using Illumina Hiseq 2500 platform. A set of telomere-related genes were found in the transcriptome, including tert, tep1, and six shelterin complex proteins coding genes (trf1, trf2, tpp1, pot1, tin2 and rap1). These genes products protect chromosome ends from deterioration and therefore significantly contribute to turtle longevity. The transcriptome data generated in this study provides a comprehensive reference for future molecular studies in the turtle.

【 授权许可】

CC BY