DNA barcode data accurately assign higher spider taxa

PeerJ

article

Jonathan A. Coddington¹  Ingi Agnarsson¹  Ren-Chung Cheng³  Klemen Čandek³  Amy Driskell¹  Holger Frick⁴  Matjaž Gregorič³  Rok Kostanjšek⁵  Christian Kropf⁴  Matthew Kweskin¹  Tjaša Lokovšek³  Miha Pipan³  Nina Vidergar³  Matjaž Kuntner¹ 

[1] National Museum of Natural History, Smithsonian Institution;Department of Biology, University of Vermont;EZ Lab, Institute of Biology, Research Centre of the Slovenian Academy of Sciences and Arts;Department of Invertebrates;Department of Biology, Biotechnical Faculty, University of Ljubljana;Department of Biochemistry, University of Cambridge

关键词: Taxonomic impediment;    Family;    Genus;    Global Genome Initiative;    Genome;    DNA barcoding;   

DOI  :  10.7717/peerj.2201

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

95 and families at PIdent values ≥ 91, suggesting these as heuristic thresholds for accurate generic and familial identifications in spiders. Accuracy of identification increases with numbers of species/genus and genera/family in the library; above five genera per family and fifteen species per genus all higher taxon assignments were correct. We propose that using percent sequence identity between conventional barcode sequences may be a feasible and reasonably accurate method to identify animals to family/genus. However, the quality of the underlying database impacts accuracy of results; many outliers in our dataset could be attributed to taxonomic and/or sequencing errors in BOLD and GenBank. It seems that an accurate and complete reference library of families and genera of life could provide accurate higher level taxonomic identifications cheaply and accessibly, within years rather than decades.

【 授权许可】

CC BY