Microsatellite loci in the tiger shark and cross-species amplification using pyrosequencing technology

PeerJ

article

Natália J. Mendes¹  Vanessa P. Cruz¹  Fernando Y. Ashikaga¹  Sâmia M. Camargo¹  Claudio Oliveira¹  Andrew N. Piercy³  George H. Burgess⁴  Rui Coelho⁵  Miguel N. Santos⁵  Fernando F. Mendonça²  Fausto Foresti¹ 

[1] Laboratório de Biologia e Genética de Peixes, Universidade Estadual Paulista;Laboratório de Genética Pesqueira e Conservação, Institute of Marine Sciences, Universidade Federal de São Paulo;Lake Nona Campus, Valencia College;Florida Museum of Natural History, Florida Program for Shark Research, University of Florida;IPMA, Portuguese Institute for the Ocean and Atmosphere

关键词: High-throughput sequencing;    Galeocerdo cuvier;    Shark;    Microsatellites;    Population structure;   

DOI  :  10.7717/peerj.2205

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

The tiger shark (Galeocerdo cuvier) has a global distribution in tropical and warm temperate seas, and it is caught in numerous fisheries worldwide, mainly as bycatch. It is currently assessed as near threatened by the International Union for Conservation of Nature (IUCN) Red List. In this study, we identified nine microsatellite loci through next generation sequencing (454 pyrosequencing) using 29 samples from the western Atlantic. The genetic diversity of these loci were assessed and revealed a total of 48 alleles ranging from 3 to 7 alleles per locus (average of 5.3 alleles). Cross-species amplification was successful at most loci for other species such as Carcharhinus longimanus, C. acronotus and Alopias superciliosus. Given the potential applicability of genetic markers for biological conservation, these data may contribute to the population assessment of this and other species of sharks worldwide.

【 授权许可】

CC BY