Accuracy of conventional identification methods used for Enterobacteriaceae isolates in three Nigerian hospitals

PeerJ

article

Christiana Jesumirhewe¹  Peter Oladejo Ogunlowo¹  Mitsan Olley²  Burkhard Springer³  Franz Allerberger³  Werner Ruppitsch³ 

[1] Department of Pharmaceutical Microbiology, Igbinedion University Okada;Department of Medical Microbiology, Igbinedion Univesity Teaching Hospital;Austrian Agency for Health and Food Safety ,(AGES), Institute of Medical Microbiology and Hygiene

关键词: Enterobacteriaceae;    Bacterial identification;    MALDI-TOF MS;   

DOI  :  10.7717/peerj.2511

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundEnterobacteriaceae are ubiquitously present in nature and can be found in the intestinal tract of humans and animals as commensal flora. Multidrug-resistant Enterobacteriaceae are increasingly reported and are a threat to public health implicating a need for accurate identification of the isolates to species level. In developing countries, identification of bacteria basically depends on conventional methods: culture and phenotypic methods that hamper the accurate identification of bacteria. In this study, matrix-assisted desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) technique was compared to conventional identification techniques.Materials and MethodsIn total, 147 Enterobacteriaceae isolates were collected from March to May 2015 from three medical microbiology laboratories of hospitals in Edo state, Nigeria, after being tested according to the individual laboratories standard operating procedures. All isolates were stored at −20°C until tested centrally by MALDI-TOF MS.Resultsor =2.0, indicating a secure genus and probable species identification; and 2(1.36%) isolates had a best score or =2.0.ConclusionThe results of our study show that the applied conventional identification techniques for Enterobacteriaceae in the investigated Nigerian hospitals are not very accurate. Use of state-of-the-art identification technologies for microorganisms is necessary to guarantee comparability of bacteriological results.

【 授权许可】

CC BY