YUCCA auxin biosynthetic genes are required for Arabidopsis shade avoidance

PeerJ

article

Patricia Müller-Moulé¹  Kazunari Nozue¹  Melissa L. Pytlak¹  Christine M. Palmer¹  Michael F. Covington¹  Andreah D. Wallace¹  Stacey L. Harmer¹  Julin N. Maloof¹ 

[1] Department of Plant Biology, University of California;PASCO Scientific;Natural Sciences Department, Castleton University;Amaryllis Nucleics;Clontech Laboratories

关键词: Auxin;    Shade avoidance;    Phytochrome;    Photomorphogenesis;   

DOI  :  10.7717/peerj.2574

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Plants respond to neighbor shade by increasing stem and petiole elongation. Shade, sensed by phytochrome photoreceptors, causes stabilization of PHYTOCHROME INTERACTING FACTOR proteins and subsequent induction of YUCCA auxin biosynthetic genes. To investigate the role of YUCCA genes in phytochrome-mediated elongation, we examined auxin signaling kinetics after an end-of-day far-red (EOD-FR) light treatment, and found that an auxin responsive reporter is rapidly induced within 2 hours of far-red exposure. YUCCA2, 5, 8, and 9 are all induced with similar kinetics suggesting that they could act redundantly to control shade-mediated elongation. To test this hypothesis we constructed a yucca2, 5, 8, 9 quadruple mutant and found that the hypocotyl and petiole EOD-FR and shade avoidance responses are completely disrupted. This work shows that YUCCA auxin biosynthetic genes are essential for detectable shade avoidance and that YUCCA genes are important for petiole shade avoidance.

【 授权许可】

CC BY