Whole-brain ex-vivo quantitative MRI of the cuprizone mouse model

PeerJ

article

Tobias C. Wood¹  Camilla Simmons¹  Samuel A. Hurley²  Anthony C. Vernon⁴  Joel Torres¹  Flavio Dell’Acqua¹  Steve C.R. Williams¹  Diana Cash¹ 

[1]Department of Neuroimaging, IOPPN, King’s College London

[2]FMRIB Centre, Nuffield Department of Clinical Neurosciences, University of Oxford

[3]Synaptive Medical

[4]Cells and Behaviour Unit, Department of Basic and Clinical Neuroscience, IOPPN, King’s College London

[5]NatBrainLab, Department of Basic and Clinical Neuroscience, IOPPN, King’s College London

关键词: Cuprizone;    Myelin;    Quantitative imaging;    MRI;    Inflammation;    Mouse;   

DOI  :  10.7717/peerj.2632

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Myelin is a critical component of the nervous system and a major contributor to contrast in Magnetic Resonance (MR) images. However, the precise contribution of myelination to multiple MR modalities is still under debate. The cuprizone mouse is a well-established model of demyelination that has been used in several MR studies, but these have often imaged only a single slice and analysed a small region of interest in the corpus callosum. We imaged and analyzed the whole brain of the cuprizone mouse ex-vivo using high-resolution quantitative MR methods (multi-component relaxometry, Diffusion Tensor Imaging (DTI) and morphometry) and found changes in multiple regions, including the corpus callosum, cerebellum, thalamus and hippocampus. The presence of inflammation, confirmed with histology, presents difficulties in isolating the sensitivity and specificity of these MR methods to demyelination using this model.

【 授权许可】

CC BY