PeerJ | |
Whole-brain ex-vivo quantitative MRI of the cuprizone mouse model | |
article | |
Tobias C. Wood1  Camilla Simmons1  Samuel A. Hurley2  Anthony C. Vernon4  Joel Torres1  Flavio Dell’Acqua1  Steve C.R. Williams1  Diana Cash1  | |
[1] Department of Neuroimaging, IOPPN, King’s College London;FMRIB Centre, Nuffield Department of Clinical Neurosciences, University of Oxford;Synaptive Medical;Cells and Behaviour Unit, Department of Basic and Clinical Neuroscience, IOPPN, King’s College London;NatBrainLab, Department of Basic and Clinical Neuroscience, IOPPN, King’s College London | |
关键词: Cuprizone; Myelin; Quantitative imaging; MRI; Inflammation; Mouse; | |
DOI : 10.7717/peerj.2632 | |
学科分类:社会科学、人文和艺术(综合) | |
来源: Inra | |
【 摘 要 】
Myelin is a critical component of the nervous system and a major contributor to contrast in Magnetic Resonance (MR) images. However, the precise contribution of myelination to multiple MR modalities is still under debate. The cuprizone mouse is a well-established model of demyelination that has been used in several MR studies, but these have often imaged only a single slice and analysed a small region of interest in the corpus callosum. We imaged and analyzed the whole brain of the cuprizone mouse ex-vivo using high-resolution quantitative MR methods (multi-component relaxometry, Diffusion Tensor Imaging (DTI) and morphometry) and found changes in multiple regions, including the corpus callosum, cerebellum, thalamus and hippocampus. The presence of inflammation, confirmed with histology, presents difficulties in isolating the sensitivity and specificity of these MR methods to demyelination using this model.
【 授权许可】
CC BY
【 预 览 】
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RO202307100014676ZK.pdf | 4867KB | download |