Identification of phenolic secondary metabolites from Schotia brachypetala Sond. (Fabaceae) and demonstration of their antioxidant activities in Caenorhabditis elegans

PeerJ

article

Mansour Sobeh¹  Esraa ElHawary²  Herbenya Peixoto¹  Rola M. Labib²  Heba Handoussa³  Noha Swilam⁴  Ahmed H. El-Khatib⁵  Farukh Sharapov¹  Tamer Mohamed¹  Sonja Krstin¹  Michael W. Linscheid⁵  Abdel Nasser Singab²  Michael Wink¹  Nahla Ayoub² 

[1] Institute of Pharmacy and Molecular Biotechnology, Heidelberg University;Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University;Department of Pharmaceutical Biology, Faculty of Pharmacy and Biotechnology, German University in Cairo;Department of Pharmacognosy, Faculty of Pharmacy, British University in Egypt;Department of Chemistry, Humboldt Universität Berlin;Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University;Department of Pharmacology and Toxicology, Faculty of Medicine, Umm Al-Qura University

关键词: Polyphenolics;    LC/HRESI/MS/MS;    Caenorhabditis elegans;    Antioxidant activity.;    Schotia brachypetala;   

DOI  :  10.7717/peerj.2404

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundSchotia brachypetala Sond. (Fabaceae) is an endemic tree of Southern Africa whose phytochemistry and pharmacology were slightly studied. The present work aimed at profiling the major phenolics compounds present in the hydro-alcohol extract from S. brachypetala leaves (SBE) using LC/HRESI/MS/MS and NMR and prove their antioxidant capabilities using novel methods.MethodsIn vitro assays; DPPH, TEAC persulfate decolorizing kinetic and FRAP assays, and in vivo assays: Caenorhabditis elegans strains maintenance, Intracellular ROS in C. elegans, Survival assay, GFP expression and Subcellular DAF-16 localization were employed to evaluate the antioxidant activity.ResultsMore than forty polyphenols, including flavonoid glycosides, galloylated flavonoid glycosides, isoflavones, dihydrochalcones, procyanidins, anthocyanins, hydroxy benzoic acid derivatives, hydrolysable tannins, and traces of methylated and acetylated flavonoid derivatives were identified. Three compounds were isolated and identified from the genus Schotia for the first time, namely gallic acid, myricetin-3-O-α-L-1C4-rhamnoside and quercetin-3-O-L-1C4-rhamnoside. The total phenolics content of SBE was (376 mg CAE/g), followed by flavonoids (67.87 QE/g). In vitro antioxidant activity of SBE was evidenced by DPPH radical scavenging activity (IC50 of 9 µg/mL), FRAP ferric reducing activity (5,000 mol Fe2+ E/mg) and ABTS peroxide inhibiting activity (1,054 mM Trolox E/mg). The tested extract was able to protect the worms against juglone induced oxidative stress, an increased survival rate (up to 41%) was recorded, when compared with the control group (11%) and attenuate the reactive oxygen species (ROS) accumulation in dose-dependent and reached up to 72% for the highest tested concentration. SBE was also able to attenuate the levels of heat shock protein (HSP) expression in dose-dependent up to 60% in the 150 µg SBE/mL group. In DAF-16 Subcellular localization SBE treated worms showed nuclear localization pattern up to 78%, while it was only 5% in the untreated control group.DiscussionA pronounced antioxidant activity in vivo, which can be attributed to its ability to promote the nuclear translocation of DAF-16/FOXO, the main transcription factor regulating the expression of stress response genes. The remarkable antioxidant activity in vitro and in vivo correlates to SBE rich phenolic profile.

【 授权许可】

CC BY