­Genomic data mining of the marine actinobacteria Streptomyces sp. H-KF8 unveils insights into multi-stress related genes and metabolic pathways involved in antimicrobial synthesis

PeerJ

article

Agustina Undabarrena¹  Juan A. Ugalde²  Michael Seeger¹  Beatriz Cámara¹ 

[1] Departmento de Química & Centro de Biotecnología, Universidad Técnica Federico Santa María;Centro de Genética y Genómica, Facultad de Medicina Clinica Alemana, Universidad del Desarrollo

关键词: Marine actinomycete;    Genome mining;    Streptomyces;    Biosynthethic gene clusters;    Antimicrobial activity;    Heavy metal tolerance;    Abiotic stressors;    Chilean Patagonian fjord;   

DOI  :  10.7717/peerj.2912

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Streptomyces sp. H-KF8 is an actinobacterial strain isolated from marine sediments of a Chilean Patagonian fjord. Morphological characterization together with antibacterial activity was assessed in various culture media, revealing a carbon-source dependent activity mainly against Gram-positive bacteria (S. aureus and L. monocytogenes). Genome mining of this antibacterial-producing bacterium revealed the presence of 26 biosynthetic gene clusters (BGCs) for secondary metabolites, where among them, 81% have low similarities with known BGCs. In addition, a genomic search in Streptomyces sp. H-KF8 unveiled the presence of a wide variety of genetic determinants related to heavy metal resistance (49 genes), oxidative stress (69 genes) and antibiotic resistance (97 genes). This study revealed that the marine-derived Streptomyces sp. H-KF8 bacterium has the capability to tolerate a diverse set of heavy metals such as copper, cobalt, mercury, chromate and nickel; as well as the highly toxic tellurite, a feature first time described for Streptomyces. In addition, Streptomyces sp. H-KF8 possesses a major resistance towards oxidative stress, in comparison to the soil reference strain Streptomyces violaceoruber A3(2). Moreover, Streptomyces sp. H-KF8 showed resistance to 88% of the antibiotics tested, indicating overall, a strong response to several abiotic stressors. The combination of these biological traits confirms the metabolic versatility of Streptomyces sp. H-KF8, a genetically well-prepared microorganism with the ability to confront the dynamics of the fjord-unique marine environment.

【 授权许可】

CC BY