ToxGen: an improved reference database for the identification of type B-trichothecene genotypes in Fusarium

PeerJ

article

Tomasz Kulik¹  Kessy Abarenkov²  Maciej Buśko³  Katarzyna Bilska¹  Anne D. van Diepeningen⁴  Anna Ostrowska-Kołodziejczak³  Katarzyna Krawczyk¹  Balázs Brankovics⁴  Sebastian Stenglein⁶  Jakub Sawicki¹  Juliusz Perkowski³ 

[1] Department of Botany and Nature Protection, University of Warmia and Mazury;Natural History Museum, University of Tartu;Department of Chemistry, Poznań University of Life Sciences;CBS-KNAW Fungal Biodiversity Centre;Institute of Biodiversity and Ecosystem Dynamics, University of Amsterdam;Laboratorio de Biología Funcional y Biotecnología ,(BIOLAB)-CICBA-INBIOTEC;Cátedra de Microbiología-Facultad de Agronomía de Azul-UNCPBA;Department of Biology and Ecology, University of Ostrava

关键词: Trichothecene genotypes;    Fusarium;    Chemotypes;    Molecular identification;    Annotation;   

DOI  :  10.7717/peerj.2992

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Type B trichothecenes, which pose a serious hazard to consumer health, occur worldwide in grains. These mycotoxins are produced mainly by three different trichothecene genotypes/chemotypes: 3ADON (3-acetyldeoxynivalenol), 15ADON (15-acetyldeoxynivalenol) and NIV (nivalenol), named after these three major mycotoxin compounds. Correct identification of these genotypes is elementary for all studies relating to population surveys, fungal ecology and mycotoxicology. Trichothecene producers exhibit enormous strain-dependent chemical diversity, which may result in variation in levels of the genotype’s determining toxin and in the production of low to high amounts of atypical compounds. New high-throughput DNA-sequencing technologies promise to boost the diagnostics of mycotoxin genotypes. However, this requires a reference database containing a satisfactory taxonomic sampling of sequences showing high correlation to actually produced chemotypes. We believe that one of the most pressing current challenges of such a database is the linking of molecular identification with chemical diversity of the strains, as well as other metadata. In this study, we use the Tri12 gene involved in mycotoxin biosynthesis for identification of Tri genotypes through sequence comparison. Tri12 sequences from a range of geographically diverse fungal strains comprising 22 Fusarium species were stored in the ToxGen database, which covers descriptive and up-to-date annotations such as indication on Tri genotype and chemotype of the strains, chemical diversity, information on trichothecene-inducing host, substrate or media, geographical locality, and most recent taxonomic affiliations. The present initiative bridges the gap between the demands of comprehensive studies on trichothecene producers and the existing nucleotide sequence databases, which lack toxicological and other auxiliary data. We invite researchers working in the fields of fungal taxonomy, epidemiology and mycotoxicology to join the freely available annotation effort.

【 授权许可】

CC BY