Grape seed proanthocyanidin extract protects lymphocytes against histone-induced apoptosis

PeerJ

article

Ping Chang¹  Bing Mo¹  David M. Cauvi²  Ying Yu¹  Zhenhui Guo³  Jian Zhou¹  Qiong Huang¹  Qitao Yan³  Guiming Chen¹  Zhanguo Liu¹ 

[1] Department of ICU, Zhujiang Hospital of Southern Medical University;Department of Surgery, University of California San Diego;Department of MICU, General Hospital of Guangzhou Military Command

关键词: Grape seed proanthocyanidin extract;    Lymphocyte apoptosis;    Mitochondrial injury;    Histones;    Reactive oxygen species;   

DOI  :  10.7717/peerj.3108

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Apoptosis of lymphocytes is associated with immunosuppression and poor prognosis in sepsis. Our previous report showed that histones, nuclear proteins released from damaged or dying cells in sepsis, can mediate lymphocyte apoptosis via mitochondria damage. Grape seed proanthocyanidin extract (GSPE), a natural substance with protective properties against oxidative stress, plays a vital role in cell and mitochondria protection. We thus hypothesized that GSPE may play a protective role in histone-induced lymphocyte apoptosis through its anti-oxidative properties. In this study, we investigated the protective efficacy of GSPE on lymphocyte apoptosis induced by extracellular histones, a main contributor of death in sepsis. Human blood lymphocytes were treated with 50 μg/ml histones, 2 μg/ml GSPE, or a combination of both. A total of 100 μM N-acetylcysteine (NAC), a reactive oxygen species (ROS) inhibitor, was used as a positive control for GSPE. Apoptosis, intracellular ROS levels, mitochondrial membrane potential, Bcl-2 expression, and caspase-3 cleavage were measured. Our data clearly indicate that GSPE significantly inhibited lymphocyte apoptosis, generation of ROS, the loss of mitochondrial membrane potential, the decrease in Bcl-2 expression, and caspase-3 activation induced by extracellular histones. In conclusion, we show that GSPE has a protective effect on lymphocyte apoptosis induced by extracellular histones. This study suggests GSPE as a potential therapeutic agent that could help reduce lymphocyte apoptosis, and thus the state of immunosuppression was observed in septic patients.

【 授权许可】

CC BY