Putative archaeal viruses from the mesopelagic ocean

PeerJ

article

Dean R. Vik¹  Simon Roux¹  Jennifer R. Brum¹  Ben Bolduc¹  Joanne B. Emerson¹  Cory C. Padilla²  Frank J. Stewart²  Matthew B. Sullivan¹ 

[1] Department of Microbiology, Ohio State University;Department of Biological Sciences, Georgia Institute of Technology;Department of Civil, Environmental and Geodetic Engineering, Ohio State University

关键词: Oxygen Minimum Zone;    Archaeal Virus;    Archaea;    Oxygen;    Temperature;    Thermoplasmata;   

DOI  :  10.7717/peerj.3428

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

99% accurate and 100% sensitive in identifying the 127 known archaeal viruses among the 12,499 viruses in the VirSorter curated dataset. Application of MArVD to 10 viral metagenomes from two depth profiles in the Eastern Tropical North Pacific (ETNP) oxygen minimum zone revealed 43 new putative archaeal virus genomes and large genome fragments ranging in size from 10 to 31 kb. Network-based classifications, which were consistent with marker gene phylogenies where available, suggested that these putative archaeal virus contigs represented six novel candidate genera. Ecological analyses, via fragment recruitment and ordination, revealed that the diversity and relative abundances of these putative archaeal viruses were correlated with oxygen concentration and temperature along two OMZ-spanning depth profiles, presumably due to structuring of the host Archaea community. Peak viral diversity and abundances were found in surface waters, where Thermoplasmata 16S rRNA genes are prevalent, suggesting these archaea as hosts in the surface habitats. Together these findings provide a baseline for identifying archaeal viruses in sequence datasets, and an initial picture of the ecology of such viruses in non-extreme environments.

【 授权许可】

CC BY