In vivo function of Pgβglu-1 in the release of acetophenones in white spruce

PeerJ

article

Melissa H. Mageroy¹  Denis Lachance³  Sharon Jancsik¹  Geneviève Parent⁴  Armand Séguin³  John Mackay⁴  Joerg Bohlmann¹ 

[1] Michael Smith Laboratories, University of British Columbia;Norwegian Institute of Bioeconomy Research;Laurentian Forestry Centre;Department of Wood and Forest Sciences, Laval University;Department of Plant Sciences, University of Oxford;Department of Forest and Conservation Sciences, University of British Columbia;Department of Botany, University of British Columbia

关键词: Picea glauca;    Conifer defense;    Spruce budworm;    Beta-glucosidase;    Transgenic spruce;   

DOI  :  10.7717/peerj.3535

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Eastern spruce budworm (Choristoneura fumiferiana Clemens) (ESBW) is a major forest pest which feeds on young shoots of white spruce (Picea glauca) and can cause landscape level economic and ecological losses. Release of acetophenone metabolites, piceol and pungenol, from their corresponding glycosides, picein and pungenin, can confer natural resistance of spruce to ESBW. A beta-glucosidase gene, Pgβglu-1, was recently discovered and the encoded enzyme was characterized in vitro to function in the release of the defensive acetophenone aglycons. Here we describe overexpression of Pgβglu-1 in a white spruce genotype whose metabolome contains the glucosylated acetophenones, but no detectable amounts of the aglycons. Transgenic overexpression of Pgβglu-1 resulted in release of the acetophenone aglycons in planta. This work provides in vivo evidence for the function of Pgβglu-1.

【 授权许可】

CC BY