Genome-wide identification and characterization of aquaporin gene family in Beta vulgaris

PeerJ

article

Weilong Kong¹  Shaozong Yang¹  Yulu Wang¹  Mohammed Bendahmane²  Xiaopeng Fu¹ 

[1] College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Key Laboratory of Horticultural Plant Biology, Ministry of Education;INRA-CNRS-Lyon1-ENS, Laboratoire Reproduction et Developpement des Plantes

关键词: Gene structure;    Expression profile;    Beta vulgaris;    Aquaporins;    Abiotic stress;   

DOI  :  10.7717/peerj.3747

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Aquaporins (AQPs) are essential channel proteins that execute multi-functions throughout plant growth and development, including water transport, uncharged solutes uptake, stress response, and so on. Here, we report the first genome-wide identification and characterization AQP (BvAQP) genes in sugar beet (Beta vulgaris), an important crop widely cultivated for feed, for sugar production and for bioethanol production. Twenty-eight sugar beet AQPs (BvAQPs) were identified and assigned into five subfamilies based on phylogenetic analyses: seven of plasma membrane (PIPs), eight of tonoplast (TIPs), nine of NOD26-like (NIPs), three of small basic (SIPs), and one of x-intrinsic proteins (XIPs). BvAQP genes unevenly mapped on all chromosomes, except on chromosome 4. Gene structure and motifs analyses revealed that BvAQP have conserved exon-intron organization and that they exhibit conserved motifs within each subfamily. Prediction of BvAQPs functions, based on key protein domains conservation, showed a remarkable difference in substrate specificity among the five subfamilies. Analyses of BvAQPs expression, by mean of RNA-seq, in different plant organs and in response to various abiotic stresses revealed that they were ubiquitously expressed and that their expression was induced by heat and salt stresses. These results provide a reference base to address further the function of sugar beet aquaporins and to explore future applications for plants growth and development improvements as well as in response to environmental stresses.

【 授权许可】

CC BY