Rootstock influence on iron uptake responses in Citrus leaves and their regulation under the Fe paradox effect

PeerJ

article

Mary-Rus Martinez-Cuenca¹  Amparo Primo-Capella¹  Ana Quiñones²  Almudena Bermejo¹  Maria Angeles Forner-Giner¹ 

[1] Centre of Citriculture and Plant Production, Valencian Agricultural and Research Institute;Centre of Sustainable Agricultural Development, Valencian Agricultural and Research Institute

关键词: Fe2+;    Ferric chelate reductase;    Organic acids;    Iron transporter;    LMWOA;    H+-ATPase;    Apoplast;    Fe active;    Rootstock;   

DOI  :  10.7717/peerj.3553

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Background and aims This work evaluates the regulation of iron uptake responses in Citrus leaves and their involvement in the Fe paradox effect. Methods Experiments were performed in field-grown ‘Navelina’ trees grafted onto two Cleopatra mandarin × Poncirus trifoliata (L.) Raf. hybrids with different Fe-chlorosis symptoms: 030146 (non-chlorotic) and 030122 (chlorotic). Results Chlorotic leaves were smaller than non-chlorotic ones for both dry weight (DW) and area basis, and exhibited marked photosynthetic state affection, but reduced catalase and peroxidase enzymatic activities. Although both samples had a similar total Fe concentration on DW, it was lower in chlorotic leaves when expressed on an area basis. A similar pattern was observed for the total Fe concentration in the apoplast and cell sap and in active Fe (Fe2+) concentration. FRO2 gene expression and ferric chelate reductase (FC-R) activity were also lower in chlorotic samples, while HA1 and IRT1 were more induced. Despite similar apoplasmic pH, K+/Ca2+ was higher in chlorotic leaves, and both citrate and malate concentrations in total tissue and apoplast fluid were lower. Conclusion (1) The rootstock influences Fe acquisition system in the leaf; (2) the increased sensitivity to Fe-deficiency as revealed by chlorosis and decreased biomass, was correlated with lower FC-R activity and lower organic acid level in leaf cells, which could cause a decreased Fe mobility and trigger other Fe-stress responses in this organ to enhance acidification and Fe uptake inside cells; and (3) the chlorosis paradox phenomenon in citrus likely occurs as a combination of a marked FC-R activity impairment in the leaf and the strong growth inhibition in this organ.

【 授权许可】

CC BY