Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe

PeerJ

article

Peter Shum¹  Lauren Moore²  Christophe Pampoulie³  Cristina Di Muri²  Sara Vandamme²  Stefano Mariani² 

[1] Hopkins Marine Station, Stanford University;School of Environment & Life Sciences, University of Salford;Marine and Freshwater Research Institute;Evolutionary and Environmental Genomics Group, School of Environmental Sciences, University of Hull;North Western Waters Advisory Council

关键词: Sebastes;    Redfish;    Barcoding;    Mislabelling;    Seafood;    COI;    Control region;   

DOI  :  10.7717/peerj.3746

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Morphology-based identification of North Atlantic Sebastes has long been controversial and misidentification may produce misleading data, with cascading consequences that negatively affect fisheries management and seafood labelling. North Atlantic Sebastes comprises of four species, commonly known as ‘redfish’, but little is known about the number, identity and labelling accuracy of redfish species sold across Europe. We used a molecular approach to identify redfish species from ‘blind’ specimens to evaluate the performance of the Barcode of Life (BOLD) and Genbank databases, as well as carrying out a market product accuracy survey from retailers across Europe. The conventional BOLD approach proved ambiguous, and phylogenetic analysis based on mtDNA control region sequences provided a higher resolution for species identification. By sampling market products from four countries, we found the presence of two species of redfish (S. norvegicus and S. mentella) and one unidentified Pacific rockfish marketed in Europe. Furthermore, public databases revealed the existence of inaccurate reference sequences, likely stemming from species misidentification from previous studies, which currently hinders the efficacy of DNA methods for the identification of Sebastes market samples.

【 授权许可】

CC BY