Formoterol attenuates increased oxidative stress and myosin protein loss in respiratory and limb muscles of cancer cachectic rats

PeerJ

article

Anna Salazar-Degracia¹  Sílvia Busquets²  Josep M. Argilés²  Francisco J. López-Soriano²  Esther Barreiro¹ 

[1] Pulmonology Department-Muscle Wasting and Cachexia in Chronic Respiratory Diseases and Lung Cancer Research Group, Health and Experimental Sciences Department ,(CEXS), IMIM-Hospital del Mar, Parc de Salut Mar, Universitat Pompeu Fabra ,(UPF), Barcelona Biomedical Research Park;Cancer Research Group, Departament de Bioquímica i Biomedicina Molecular, Facultat de Biologia, Universitat de Barcelona, Universitat de Barcelona;Institut de Biomedicina de la Universitat de Barcelona;Centro de Investigación en Red de Enfermedades Respiratorias ,(CIBERES), Instituto de Salud Carlos III

关键词: Experimental cancer-induced cachexia;    Diaphragm and gastrocnemius;    Formoterol treatment;    Redox balance;    Contractile proteins;   

DOI  :  10.7717/peerj.4109

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Muscle mass loss and wasting are characteristic features of patients with chronic conditions including cancer. Therapeutic options are still scarce. We hypothesized that cachexia-induced muscle oxidative stress may be attenuated in response to treatment with beta2-adrenoceptor-selective agonist formoterol in rats. In diaphragm and gastrocnemius of tumor-bearing rats (108 AH-130 Yoshida ascites hepatoma cells inoculated intraperitoneally) with and without treatment with formoterol (0.3 mg/kg body weight/day for seven days, daily subcutaneous injection), redox balance (protein oxidation and nitration and antioxidants) and muscle proteins (1-dimensional immunoblots), carbonylated proteins (2-dimensional immunoblots), inflammatory cells (immunohistochemistry), and mitochondrial respiratory chain (MRC) complex activities were explored. In the gastrocnemius, but not the diaphragm, of cancer cachectic rats compared to the controls, protein oxidation and nitration levels were increased, several functional and structural proteins were carbonylated, and in both study muscles, myosin content was reduced, inflammatory cell counts were greater, while no significant differences were seen in MRC complex activities (I, II, and IV). Treatment of cachectic rats with formoterol attenuated all the events in both respiratory and limb muscles. In this in vivo model of cancer-cachectic rats, the diaphragm is more resistant to oxidative stress. Formoterol treatment attenuated the rise in oxidative stress in the limb muscles, inflammatory cell infiltration, and the loss of myosin content seen in both study muscles, whereas no effects were observed in the MRC complex activities. These findings have therapeutic implications as they demonstrate beneficial effects of the beta2 agonist through decreased protein oxidation and inflammation in cachectic muscles, especially the gastrocnemius.

【 授权许可】

CC BY