Comparative genomic analysis of a new tellurite-resistant Psychrobacter strain isolated from the Antarctic Peninsula

PeerJ

article

Eduardo Hugo Morales¹  Mauricio Arenas-Salinas²  Felipe Alejandro Arenas¹  Eduardo Castro-Nallar³  Claudio Christian Vásquez¹  Claudia Melissa Muñoz-Villagrán¹  Katterinne N. Mendez³  Fabian Cornejo¹  Maximiliano Figueroa¹  Agustina Undabarrena⁵ 

[1] Laboratorio de Microbiología Molecular, Departamento de Biología, Universidad de Santiago de Chile;Centro de Bioinformática y Simulación Molecular, Universidad de Talca;Center for Bioinformatics and Integrative Biology, Facultad de Ciencias Biológicas, Universidad Andrés Bello;Departamento de Ciencias Básicas, Facultad de Ciencia, Universidad Santo Tomas Sede Santiago;Laboratorio de Microbiología Molecular y Biotecnología Ambiental, Departamento de Química & Centro de Biotecnología Daniel Alkalay Lowitt, Universidad Técnica Federico Santa María

关键词: Ter genes;    Antarctica;    Extremophiles;    Tellurite resistance;    Phylogenomics;   

DOI  :  10.7717/peerj.4402

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

The Psychrobacter genus is a cosmopolitan and diverse group of aerobic, cold-adapted, Gram-negative bacteria exhibiting biotechnological potential for low-temperature applications including bioremediation. Here, we present the draft genome sequence of a bacterium from the Psychrobacter genus isolated from a sediment sample from King George Island, Antarctica (3,490,622 bp; 18 scaffolds; G + C = 42.76%). Using phylogenetic analysis, biochemical properties and scanning electron microscopy the bacterium was identified as Psychrobacter glacincola BNF20, making it the first genome sequence reported for this species. P. glacincola BNF20 showed high tellurite (MIC 2.3 mM) and chromate (MIC 6.0 mM) resistance, respectively. Genome-wide nucleotide identity comparisons revealed that P. glacincola90%) to other uncharacterized Psychrobacter spp. such as JCM18903, JCM18902, and P11F6. Bayesian multi-locus phylogenetic analysis showed that P. glacincola BNF20 belongs to a polyphyletic clade with other bacteria isolated from polar regions. A high number of genes related to metal(loid) resistance were found, including tellurite resistance genetic determinants located in two contigs: Contig LIQB01000002.1 exhibited five ter genes, each showing putative promoter sequences (terACDEZ), whereas contig LIQB1000003.2 showed a variant of the terZ gene. Finally, investigating the presence and taxonomic distribution of ter genes in the NCBI’s RefSeq bacterial database (5,398 genomes, as January 2017), revealed that 2,623 (48.59%) genomes showed at least one ter gene. At the family level, most (68.7%) genomes harbored one ter gene and 15.6% exhibited five (including P. glacincola BNF20). Overall, our results highlight the diverse nature (genetic and geographic diversity) of the Psychrobacter genus, provide insights into potential mechanisms of metal resistance, and exemplify the benefits of sampling remote locations for prospecting new molecular determinants.

【 授权许可】

CC BY