Cytotoxicity and antimicrobial action of selected phytochemicals against planktonic and sessile Streptococcus mutans

PeerJ

article

Marta Ribeiro¹  Joana Malheiro¹  Liliana Grenho²  Maria Helena Fernandes²  Manuel Simões¹ 

[1] LEPABE—Department of Chemical Engineering, Faculty of Engineering—University of Porto;Laboratory for Bone Metabolism and Regeneration, Faculty of Dental Medicine, University of Porto;REQUIMTE/LAQV, University of Porto

关键词: Biofilm control;    Dental caries;    Phytochemicals;    Cytotoxicity;    Streptococcus mutans;   

DOI  :  10.7717/peerj.4872

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundDental caries remains the most prevalent and costly oral infectious disease worldwide, encouraging the search for new and more effective antimicrobials. Therefore, the aim of this work was to study the antimicrobial action of selected phytochemicals (eugenol, citronellol, sabinene hydrate, trans-cinnamaldehyde, terpineol and cinnamic acid) against Streptococcus mutans in planktonic and biofilm states as well as the cytotoxicity of these compounds.MethodsThe antibacterial activity of the selected compounds was evaluated by the determination of the minimal bactericidal concentration. The resazurin assay was used to assess the metabolic activity of sessile S. mutans. The cytotoxicity was determined using a fibroblast cell line.ResultsAmong the tested phytochemicals, citronellol, cinnamic acid and trans-cinnamaldehyde were the most effective against both planktonic and sessile S. mutans, an effect apparently related to their hydrophobic character. Additionally, these three compounds did not compromise fibroblasts cell viability.DiscussionCitronellol, cinnamic acid and trans-cinnamaldehyde demonstrated significant antimicrobial activity and low cytotoxicity proposing their potential as a novel group of therapeutic compounds to control oral infectious diseases. Moreover, their effects are particularly relevant when benchmarked against eugenol, a phytochemical commonly used for prosthodontic applications in dentistry.

【 授权许可】

CC BY