Alpha-mangostin inhibits the migration and invasion of A549 lung cancer cells

PeerJ

article

Thi Kieu Trang Phan¹  Fahimeh Shahbazzadeh¹  Thi Thu Huong Pham²  Takanori Kihara¹ 

[1] Department of Life and Environment Engineering, Faculty of Environmental Engineering, The University of Kitakyushu;The Key Laboratory of Enzyme & Protein Technology ,(KLEPT), VNU University of Science, Vietnam National University

关键词: Co-culture;    Cancer invasion;    α-mangostin;   

DOI  :  10.7717/peerj.5027

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Several studies have indicated that α-mangostin exerts anti-metastasis and anti-subsistence effects on several types of cancer cells. Especially, the anti-metastatic effect of α-mangostin on cancer cells is a prospective function in cancer treatment. However, the metastasis process is complicated, and includes migration, invasion, intravasation, and extravasation; thus, the main target of anti-metastatic effect of α-mangostin is not known. In this study, we investigated the effects of α-mangostin on the invasion, subsistence, and migration of lung cancer cells under co-culture conditions with normal cells and regular mono-culture conditions. We found that α-mangostin killed the lung cancer and normal cells in a dose-dependent manner. Furthermore, the alteration in the surface mechanical properties of cells was examined by using atomic force microscopy. Although the α-mangostin concentrations of 5 and 10 µM did not affect the short-term cell viability, they considerably decreased the Young’s modulus of lung cancer cells implying a decline in cell surface actin cytoskeletal properties. Additionally, these concentrations of α-mangostin inhibited the migration of lung cancer cells. In co-culture conditions (cancer cells with normal cells), the invasive activities of cancer cells on normal cells were discernibly observed, and was inhibited after treatment with 5 and 10 µM of α-mangostin. Taken together, α-mangostin suppressed the subsistence of lung cancer cells and displayed anti-metastatic activities by inhibiting the migration and invasion, and reducing the actin cytoskeleton of cancer cells. Our findings suggest that α-mangostin could be a potential therapeutic agent for cancer treatment.

【 授权许可】

CC BY