PeerJ | |
Effects of estrogens and antiestrogens on gonadal sex differentiation and embryonic development in the domestic fowl ( Gallus gallus domesticus ) | |
article | |
Luzie Jessl1  Rebecca Lenz1  Fabian G. Massing1  Jessica Scheider1  Jörg Oehlmann1  | |
[1] Department Aquatic Ecotoxicology, Johann Wolfgang Goethe Universität Frankfurt am Main;R-Biopharm AG;Dr. Drexler + Dr. Fecher GmbH;ERM GmbH | |
关键词: Gonad; Chicken embryo; Estrogen; Fulvestrant; Sex differentiation; Antiestrogen; 17α-ethinylestradiol; Tamoxifen; Endocrine disruption; | |
DOI : 10.7717/peerj.5094 | |
学科分类:社会科学、人文和艺术(综合) | |
来源: Inra | |
【 摘 要 】
Since it is known that environmental contaminants have the potential to cause endocrine disorders in humans and animals, there is an urgent need for in vivo tests to assess possible effects of these endocrine disrupting chemicals (EDCs). Although there is no standardized guideline, the avian embryo has proven to be particularly promising as it responds sensitively to a number of EDCs preferentially impacting the reproductive axis. In the present study we examined the effects of in ovo exposure to fulvestrant and tamoxifen as antiestrogenic model compounds and co-exposure to both substances and the potent estrogen 17α-ethinylestradiol (EE2) regarding sex differentiation and embryonic development of the domestic fowl (Gallus gallus domesticus). The substances were injected into the yolk of fertilized eggs on embryonic day 1. On embryonic day 19 sex genotype and phenotype were determined, followed by gross morphological and histological examination of the gonads. Sole EE2-treatment (20 ng/g egg) particularly affected male gonads and resulted in an increased formation of female-like gonadal cortex tissue and a reduction of seminiferous tubules. In ovo exposure to tamoxifen (0.1/1/10 µg/g egg) strongly impaired the differentiation of female gonads, led to a significant size reduction of the left ovary and induced malformations of the ovarian cortex, while fulvestrant (0.1/1/10 µg/g egg) did not affect sexual differentiation. However, both antiestrogens were able to antagonize the feminizing effects of EE2in genetic males when administered simultaneously. Since both estrogens and antiestrogens induce concentration-dependent morphological alterations of the sex organs, the chick embryo can be regarded as a promising model for the identification of chemicals with estrogenic and antiestrogenic activity.
【 授权许可】
CC BY
【 预 览 】
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