Identification of exosome-like nanoparticle-derived microRNAs from 11 edible fruits and vegetables

PeerJ

article

Juan Xiao¹  Siyuan Feng¹  Xun Wang¹  Keren Long¹  Yi Luo¹  Yuhao Wang¹  Jideng Ma¹  Qianzi Tang¹  Long Jin¹  Xuewei Li¹  Mingzhou Li¹ 

[1] Sichuan Agricultural University, Institute of Animal Genetics and Breeding, College of Animal Science and Technology;Sichuan Agricultural University, Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province

关键词: Exosome-like nanoparticles;    Cross-kingdom;    miRNAs expression profile;    Illumina sequencing;    miRNA expression profile;   

DOI  :  10.7717/peerj.5186

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Edible plant-derived exosome-like nanoparticles (EPDELNs) are novel naturally occurring plant ultrastructures that are structurally similar to exosomes. Many EPDELNs have anti-inflammatory properties. MicroRNAs (miRNAs) play a critical role in mediating physiological and pathological processes in animals and plants. Although miRNAs can be selectively encapsulated in extracellular vesicles, little is known about their expression and function in EPDELNs. In this study, we isolated nanovesicles from 11 edible fruits and vegetables and subjected the corresponding EPDELN small RNA libraries to Illumina sequencing. We identified a total of 418 miRNAs—32 to 127 per species—from the 11 EPDELN samples. Target prediction and functional analyses revealed that highly expressed miRNAs were closely associated with the inflammatory response and cancer-related pathways. The 418 miRNAs could be divided into three classes according to their EPDELN distributions: 26 “frequent” miRNAs (FMs), 39 “moderately present” miRNAs (MPMs), and 353 “rare” miRNAs (RMs). FMs were represented by fewer miRNA species than RMs but had a significantly higher cumulative expression level. Taken together, our in vitro results indicate that miRNAs in EPDELNs have the potential to regulate human mRNA.

【 授权许可】

CC BY