Effects of resveratrol, exercises and their combination on Farnesoid X receptor, Liver X receptor and Sirtuin 1 gene expression and apoptosis in the liver of elderly rats with nonalcoholic fatty liver

PeerJ

article

Amir Hajighasem¹  Parvin Farzanegi¹  Zohreh Mazaheri²  Marjan Naghizadeh¹  Ghoncheh Salehi¹ 

[1] Department of Exercise Physiology, Sari Branch, Islamic Azad University;Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University

关键词: Resveratrol;    Interval exercise;    NAFLD;    Continuous exercise;    Sirt1;    Lxr;    Fxr;   

DOI  :  10.7717/peerj.5522

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundNon-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disorder. This study aims to consider effects of resveratrol, exercise and their combination on Farnesoid X receptor (Fxr), the liver X receptor (Lxr) and Sirtuin 1 (Sirt 1) genes expression in the liver of elderly rats with NAFLD.MethodsRats with NAFLD were randomly divided into seven groups including patient, saline, resveratrol (RSV), interval exercise, continuous exercise, interval exercise + RSV and continuous exercise + RSV. Levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) in the liver tissue were measured using specific ELISA kits. A TUNEL assay kit was used for the assessment of hepatic cells apoptosis. Lipid profiles were considered by measuring the serum triglyceride, cholesterol, LDL, and HDL. Expression of Sirt1, Lxr and Fxr genes was considered using RT-PCR.ResultsResveratrol administration alone or combined with exercise training significantly improved the expression of Sirt1, Lxr and Fxr genes (p < 0.05) in the hepatic tissue of rats with NAFLD, while levels of AST, ALT, ALP enzymes, as well as apoptotic cells were significantly decreased (p < 0.05).DiscussionAlthough resveratrol alone improves the expression of Sirt1, Lxr and Fxr, as well as liver function, combined therapy with exercise training is more effective to improve NAFLD.

【 授权许可】

CC BY