A secretory hexokinase plays an active role in the proliferation of Nosema bombycis

PeerJ

article

Yukang Huang¹  Shiyi Zheng¹  Xionge Mei¹  Bin Yu¹  Bin Sun¹  Boning Li¹  Junhong Wei¹  Jie Chen¹  Tian Li¹  Guoqing Pan¹  Zeyang Zhou¹  Chunfeng Li¹ 

[1] State Key Laboratory Of Silkworm Genome Biology, Southwest University;Southwest University, Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry;Chongqing Normal University, College of Life Sciences

关键词: Microsporidia;    Hexokinase;    Secretory protein;    RNAi;   

DOI  :  10.7717/peerj.5658

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

The microsporidian Nosema bombycis is an obligate intracellular parasite of Bombyx mori, that lost its intact tricarboxylic acid cycle and mitochondria during evolution but retained its intact glycolysis pathway. N. bombycis hexokinase (NbHK) is not only a rate-limiting enzyme of glycolysis but also a secretory protein. Indirect immunofluorescence assays and recombinant HK overexpressed in BmN cells showed that NbHK localized in the nucleus and cytoplasm of host cell during the meront stage. When N. bombycis matured, NbHK tended to concentrate at the nuclei of host cells. Furthermore, the transcriptional profile of NbHK implied it functioned during N. bombycis’ proliferation stages. A knock-down of NbHK effectively suppressed the proliferation of N. bombycis indicating that NbHK is an important protein for parasite to control its host.

【 授权许可】

CC BY