Evaluation of 17 microsatellite markers for parentage testing and individual identification of domestic yak ( Bos grunniens )

PeerJ

article

Jie Pei¹  Pengjia Bao¹  Min Chu¹  Chunnian Liang¹  Xuezhi Ding¹  Hongbo Wang¹  Xiaoyun Wu¹  Xian Guo¹  Ping Yan¹ 

[1] Animal Science Department, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences;Key Laboratory for Yak Genetics, Breeding, and Reproduction Engineering of Gansu Province

关键词: Polled yak;    Simple sequence repeats;    Forensic genetics;    Genetic diversity;    Microsatellites;    Short tandem repeats;    Identification;    Parentage testing;   

DOI  :  10.7717/peerj.5946

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Background Yak (Bos grunniens) is the most important domestic animal for people living at high altitudes. Yak ordinarily feed by grazing, and this behavior impacts the accuracy of the pedigree record because it is difficult to control mating in grazing yak. This study aimed to evaluate the pedigree system and individual identification in polled yak. Methods A total of 71 microsatellite loci were selected from the literature, mostly from the studies on cattle. A total of 35 microsatellite loci generated excellent PCR results and were evaluated for the parentage testing and individual identification of 236 unrelated polled yaks. A total of 17 of these 35 microsatellite loci had polymorphic information content (PIC) values greater than 0.5, and these loci were in Hardy–Weinberg equilibrium without linkage disequilibrium. Results 0.99999999 and 0.99999899, respectively. These findings indicated that the combination of 17 microsatellite markers could be useful for efficient and reliable parentage testing and individual identification in polled yak. Discussion Many microsatellite loci have been investigated for cattle paternity testing. Nevertheless, these loci cannot be directly applied to yak identification because the two bovid species have different genomic sequences and organization. A total of 17 loci were selected from 71 microsatellite loci based on efficient amplification, unambiguous genotyping, and high PIC values for polled yaks, and were suitable for parentage analysis in polled yak populations.

【 授权许可】

CC BY