Whole-genome comparisons of Penicillium spp. reveals secondary metabolic gene clusters and candidate genes associated with fungal aggressiveness during apple fruit decay

PeerJ

article

Guangxi Wu¹  Wayne M. Jurick II²  Franz J. Lichtner¹  Hui Peng²  Guohua Yin³  Verneta L. Gaskins²  Yanbin Yin⁴  Sui-Sheng Hua⁵  Kari A. Peter⁶  Joan W. Bennett³ 

[1] Oak Ridge Institute for Science and Education;Food Quality Laboratory;Department of Plant Biology, Rutgers, The State University of New Jersey—Camden;Department of Biological Sciences, Northern Illinois University;Western Regional Research Center;Plant Pathology and Environmental Microbiology, Penn State University

关键词: Penicillium spp;    Blue mold;    Pome fruit;    Comparative genomics;    Gene profiling;   

DOI  :  10.7717/peerj.6170

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Blue mold is a postharvest rot of pomaceous fruits caused by Penicillium expansum and a number of other Penicillium species. The genome of the highly aggressive P. expansum strain R19 was re-sequenced and analyzed together with the genome of the less aggressive P. solitum strain RS1. Whole genome scale similarities and differences were examined. A phylogenetic analysis of P. expansum, P. solitum, and several closely related Penicillium species revealed that the two pathogens isolated from decayed apple with blue mold symptoms are not each other’s closest relatives. Among a total of 10,560 and 10,672 protein coding sequences respectively, a comparative genomics analysis revealed 41 genes in P. expansum R19 and 43 genes in P. solitum RS1 that are unique to these two species. These genes may be associated with pome fruit–fungal interactions, subsequent decay processes, and mycotoxin accumulation. An intact patulin gene cluster consisting of 15 biosynthetic genes was identified in the patulin producing P. expansum strain R19, while only a remnant, seven-gene cluster was identified in the patulin-deficient P. solitum strain. However, P. solitum contained a large number of additional secondary metabolite gene clusters, indicating that this species has the potential capacity to produce an array of known as well as not-yet-identified products of possible toxicological or biotechnological interest.

【 授权许可】

CC BY