Genome-wide analysis of circular RNAs in goat skin fibroblast cells in response to Orf virus infection

PeerJ

article

Feng Pang¹  Mengmeng Zhang¹  Xiaojian Yang¹  Guohua Li¹  Shu Zhu¹  Xin Nie¹  Ruiyong Cao¹  Xiaohong Yang¹  Zhenxing Zhang¹  Haifeng Huang¹  Baobao Li¹  Chengqiang Wang¹  Li Du¹  Fengyang Wang¹ 

[1] College of Animal Science and Technology, College of Tropical Agriculture and Forestry, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Hainan University

关键词: GSF cells;    Orf virus;    circRNA;    Host genes;    miRNA sponges;    ceRNA network;   

DOI  :  10.7717/peerj.6267

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Orf, caused by Orf virus (ORFV), is a globally distributed zoonotic disease responsible for serious economic losses in the agricultural sector. However, the mechanism underlying ORFV infection remains largely unknown. Circular RNAs (circRNAs), a novel type of endogenous non-coding RNAs, play important roles in various pathological processes but their involvement in ORFV infection and host response is unclear. In the current study, whole transcriptome sequencing and small RNA sequencing were performed in ORFV-infected goat skin fibroblast cells and uninfected cells. A total of 151 circRNAs, 341 messenger RNAs (mRNAs), and 56 microRNAs (miRNAs) were differently expressed following ORFV infection. Four circRNAs: circRNA1001, circRNA1684, circRNA3127 and circRNA7880 were validated by qRT-PCR and Sanger sequencing. Gene ontology (GO) analysis indicated that host genes of differently expressed circRNAs were significantly enriched in regulation of inflammatory response, epithelial structure maintenance, positive regulation of cell migration, positive regulation of ubiquitin-protein transferase activity, regulation of ion transmembrane transport, etc. The constructed circRNA-miRNA-mRNA network suggested that circRNAs may function as miRNA sponges indirectly regulating gene expression following ORFV infection. Our study presented the first comprehensive profiles of circRNAs in response to ORFV infection, thus providing new clues for the mechanisms of interactions between ORFV and the host.

【 授权许可】

CC BY