Two membrane-bound transcription factors regulate expression of various type-IV-pili surface structures in Sulfolobus acidocaldarius

PeerJ

article

Lisa Franziska Bischof¹  Maria Florencia Haurat³  Sonja-Verena Albers¹ 

[1] Institute of Biology II, Molecular Biology of Archaea, University of Freiburg;Spemann Graduate School of Biology and Medicine;Department of Molecular Microbiology, Washington University, School of Medicine in St. Louis

关键词: Transcription regulation;    One-component system;    Archaea;    Signal transduction;    Cell motility;    Membrane protein;   

DOI  :  10.7717/peerj.6459

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

In Archaea and Bacteria, gene expression is tightly regulated in response to environmental stimuli. In the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius nutrient limitation induces expression of the archaellum, the archaeal motility structure. This expression is orchestrated by a complex hierarchical network of positive and negative regulators—the archaellum regulatory network (arn). The membrane-bound one-component system ArnR and its paralog ArnR1 were recently described as main activators of archaellum expression in S. acidocaldarius. They regulate gene expression of the archaellum operon by targeting the promoter of flaB, encoding the archaellum filament protein. Here we describe a strategy for the isolation and biochemical characterization of these two archaellum regulators. Both regulators are capable of forming oligomers and are phosphorylated by the Ser/Thr kinase ArnC. Apart from binding to pflaB, ArnR but not ArnR1 bound to promoter sequences of aapF and upsX, which encode components of the archaeal adhesive pilus and UV-inducible pili system, demonstrating a regulatory connection between different surface appendages of S. acidocaldarius.

【 授权许可】

CC BY