Transcriptome analysis of osmotic-responsive genes in ABA-dependent and -independent pathways in wheat ( Triticum aestivum L.) roots

PeerJ

article

Chunxi Li¹  Wenli Zhang¹  Meng Yuan¹  Lina Jiang¹  Bo Sun¹  Daijing Zhang¹  Yun Shao¹  Anqi Liu¹  Xueqing Liu¹  Jianhui Ma¹ 

[1] College of Life Science, Henan Normal University

关键词: Triticum aestivum L.;    Osmotic stress;    Abscisic acid;    Transcriptomic;   

DOI  :  10.7717/peerj.6519

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Bread wheat is one of the most important crops in the world. However, osmotic stress significantly inhibits wheat growth and development, and reduces crop yield and quality. Plants respond to osmotic stress mainly through abscisic acid (ABA)-dependent and -independent pathways. In this study, root transcriptome profiles of wheat seedlings exposed to osmotic stress and exogenous ABA were analysed to identify osmotic-responsive genes belonging to the ABA-dependent or -independent pathways. We found that osmotic stress promoted proline biosynthesis in the ABA-dependent pathway, and trehalose biosynthesis is likely promoted among soluble sugars to maintain protein bioactivity under osmotic stress. In wheat roots subjected to osmotic stress, calcium ions, and glutathione exert their functions mainly through calcium-binding protein (CaM/CML) and glutathione-S-transferase, respectively, depending on both pathways. In addition, a complex relationship among phytohormones signal transduction was observed in response to osmotic stress. The findings of this study deepen our understanding of the molecular mechanisms of osmotic-stress resistance, and provide several candidate osmotic-responsive genes for further study.

【 授权许可】

CC BY