Acute toxicity and responses of antioxidant systems to dibutyl phthalate in neonate and adult Daphnia magna

PeerJ

article

Chenchen Shen¹  Jie Wei¹  Tianyi Wang¹  Yuan Wang¹ 

[1]Key Laboratory of Hydrobiology in Liaoning Province, Dalian Ocean University

关键词: Acute toxicity;    Antioxidant enzymes;    Lipid peroxidation;    Daphnia magna;    Dibutyl phthalate;   

DOI  :  10.7717/peerj.6584

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Dibutyl phthalate (DBP) poses a severe threat to aquatic ecosystems, introducing hazards to both aquatic species and human health. The ecotoxic effects of DBP on aquatic organisms have not been fully investigated. This study investigates acute toxicity, oxidative damage, and antioxidant enzyme parameters in neonate and adult Daphnia magna exposed to DBP. The obtained results show comparable DBP toxic responses in neonates and adults. The median lethal concentrations (LC50) of DBP in neonates exposed for 24 and 48 h were 3.48 and 2.83 mg/L, respectively. The LC50 of adults for the same DBP exposure durations were 4.92 and 4.31 mg/L, respectively. Increased hydrogen peroxide and malondialdehyde were found in neonates and adults at both 24 and 48 h, while the total antioxidant capacity decreased. Superoxide dismutase activity increased significantly in neonates and adults exposed to 0.5 mg/L DBP, and subsequently diminished at higher DBP concentrations and prolonged exposure. Catalase and glutathione S-transferases activities both decreased markedly in neonates and adults. The changes observed were found to be time and concentration dependent. Overall, these data indicated that the acute toxic effects of DBP exposure on neonates were more pronounced than in adults, and oxidative injury may be the main mechanism of DBP toxicity. These results provide a functional link for lipid peroxidation, antioxidant capacity, and antioxidant enzyme levels in the Daphnia magna response to DBP exposure.

【 授权许可】

CC BY