Associated bacteria of Botryococcus braunii (Chlorophyta)

PeerJ

article

Joao D. Gouveia¹  Jie Lian²  Georg Steinert²  Hauke Smidt²  Detmer Sipkema²  Rene H. Wijffels¹  Maria J. Barbosa¹ 

[1] Bioprocess Engineering, Wageningen University & Research;Laboratory of Microbiology, Wageningen University & Research;Faculty of Biosciences and Aquaculture, Nord University;Department of Biology, University of Bergen

关键词: Botryococcus braunii;    Associated bacteria;    Algal–bacterial interactions;    16S rRNA sequencing;   

DOI  :  10.7717/peerj.6610

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Botryococcus braunii (Chlorophyta) is a green microalga known for producing hydrocarbons and exopolysaccharides (EPS). Improving the biomass productivity of B. braunii and hence, the productivity of the hydrocarbons and of the EPS, will make B. braunii more attractive for industries. Microalgae usually cohabit with bacteria which leads to the formation of species-specific communities with environmental and biological advantages. Bacteria have been found and identified with a few B. braunii strains, but little is known about the bacterial community across the different strains. A better knowledge of the bacterial community of B. braunii will help to optimize the biomass productivity, hydrocarbons, and EPS accumulation. To better understand the bacterial community diversity of B. braunii, we screened 12 strains from culture collections. Using 16S rRNA gene analysis by MiSeq we described the bacterial diversity across 12 B. braunii strains and identified possible shared communities. We found three bacterial families common to all strains: Rhizobiaceae, Bradyrhizobiaceae, and Comamonadaceae. Additionally, the results also suggest that each strain has its own specific bacteria that may be the result of long-term isolated culture.

【 授权许可】

CC BY