Environmental modulation of the proteomic profiles from closely phylogenetically related populations of the red seaweed Plocamium brasiliense

PeerJ

article

Gabriela Calegario¹  Lucas Freitas³  Eidy Santos⁵  Bruno Silva¹  Louisi Oliveira¹  Gizele Garcia¹  Cláudia Omachi¹  Renato Pereira⁷  Cristiane Thompson¹  Fabiano Thompson¹ 

[1] Institute of Biology, Federal University of Rio de Janeiro;SAGE-COPPE, Federal University of Rio de Janeiro;Department of Biochemistry, Federal University of Rio de Janeiro;Department of Genetics, Evolution, Microbiology and Immunology, State University of Campinas;Unit of Biology, State University of the West Zone;Department of Undergraduate Education, Federal University of Rio de Janeiro;Department of Marine Biology, Fluminense Federal University;Rio de Janeiro Botanical Garden

关键词: Environmental differences;    Population genetics;    Photosynthesis;    Phylogenetics;    Proteomics;    Rhodophyta;   

DOI  :  10.7717/peerj.6469

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

The genus Plocamium encompasses seaweeds that are widely distributed throughout the world’s oceans, with Plocamium brasiliense found along the tropical and subtropical coasts of the Western Atlantic. This wide distribution can lead to structured populations due to environmental differences (e.g., light levels or temperature), restricted gene flow, and the presence of cryptic species. Abiotic variation can also affect gene expression, which consequently leads to differences in the seaweeds protein profile. This study aimed to analyze the genetic and proteomic profiles of P. brasiliense sampled in two geographically distinct sites on the coastline of Rio de Janeiro state, Brazil: Arraial do Cabo (P1) and Búzios (P2). The genetic profiles of macroalgal specimens from these two sites were indistinguishable as assessed by the markers UPA/23S, rbcL, and COI-5P; however, the protein profiles varied significantly between populations from the two sites. At both sites the ribulose-1,5-biphosphate carboxylase/oxygenase was the most abundant protein found in P. brasiliense specimens. The number of phycobiliproteins differed between both sites with the highest numbers being found at P1, possibly due to water depth. The differences in proteomic profiles of the two nearly identical populations of P. brasiliense suggest that environmental parameters such as light availability and desiccation might induce distinct protein expression, probably as a result of the phenotypic plasticity within this population of seaweed.

【 授权许可】

CC BY