Biodiversity seen through the perspective of insects: 10 simple rules on methodological choices and experimental design for genomic studies

PeerJ

article

Pável Matos-Maraví¹  Camila Duarte Ritter⁴  Christopher J. Barnes⁵  Martin Nielsen⁵  Urban Olsson¹  Niklas Wahlberg⁷  Daniel Marquina⁸  Ilari Sääksjärvi^1,10  Alexandre Antonelli¹ 

[1] Department of Biological and Environmental Sciences, University of Gothenburg;Gothenburg Global Biodiversity Centre;Institute of Entomology, Biology Centre CAS;Department of Eukaryotic Microbiology, University of Duisburg-Essen;Natural History Museum of Denmark, University of Copenhagen;Section for Evolutionary Genomics, Department of Biology, University of Copenhagen;Department of Biology, Lund University;Department of Bioinformatics and Genetics;Department of Zoology, Stockholm University;Biodiversity Unit, University of Turku;Royal Botanical Garden

关键词: Evolution;    High-throughput sequencing;    Insect genomics;    Museomics;    Taxonomic impediment;   

DOI  :  10.7717/peerj.6727

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Massively parallel DNA sequencing opens up opportunities for bridging multiple temporal and spatial dimensions in biodiversity research, thanks to its efficiency to recover millions of nucleotide polymorphisms. Here, we identify the current status, discuss the main challenges, and look into future perspectives on biodiversity genomics focusing on insects, which arguably constitute the most diverse and ecologically important group among all animals. We suggest 10 simple rules that provide a succinct step-by-step guide and best-practices to anyone interested in biodiversity research through the study of insect genomics. To this end, we review relevant literature on biodiversity and evolutionary research in the field of entomology. Our compilation is targeted at researchers and students who may not yet be specialists in entomology or molecular biology. We foresee that the genomic revolution and its application to the study of non-model insect lineages will represent a major leap to our understanding of insect diversity.

【 授权许可】

CC BY