Chemovariation and antibacterial activity of extracts and isolated compounds from species of Ixora and Greenea (Ixoroideae, Rubiaceae)

PeerJ

article

Raveevatoo Buathong¹  Voradol Chamchumroon²  Johann Schinnerl³  Markus Bacher⁴  Wichai Santimaleeworagun⁵  Ekaphan Kraichak¹  Srunya Vajrodaya¹ 

[1] Department of Botany, Faculty of Science, Kasetsart University;The Forest Herbarium, National Parks, Wildlife and Plant Conservation Department;Chemodiversity Research Group, Department of Botany and Biodiversity Research, University of Vienna;Institute of Chemistry of Renewable Resources, University of Natural Resources and Life Sciences Vienna;Department of Pharmacy, Faculty of Pharmacy, Silpakorn University;Novel Antibiotic Compound Project by Pharmaceutical Initiative for Resistant Bacteria and Infectious Diseases Working Group ,(PIRBIG), Silpakorn University

关键词: Antibacterials;    Ixora;    Greenea;    Scopoletin;    Geniposidic acid;   

DOI  :  10.7717/peerj.6893

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Background A large number of secondary metabolites can be obtained from plants used for traditional medicine in two related genera (Ixora and Greenea) in the subfamily Ixoroideae (Rubiaceae), but there are only a few detailed studies on their bioactivities. Therefore, the main goals of this study were to determine the antibacterial activities of lipophilic extracts from plants of some Ixora and Greenea species native to Thailand, and to isolate some pure compounds from those extracts. Moreover, we compared the occurrence of compounds in different plant parts of samples from different habitats to better understand their variation. Methods A total of 56 lipophilic extracts were obtained from the leaves, stem bark, and root bark of eight Ixora and two Greenea species collected at various locations in Thailand. Isolated compounds were identified using nuclear magnetic resonance. Antimicrobial activities were evaluated against four Gram-positive and nine Gram-negative human pathogenic bacterial strains. Results Extracts from I. javanica, I. nigricans, I. brunonis, and G. montana, along with isolated scopoletin, exhibited antibacterial activities against Gram-positive methicillin-resistant Staphylococcus aureus ATCC 43300, with minimum inhibitory concentration values ranging from 64 to 256 µg/mL. The occurrence of scopoletin, isofraxidin, and geniposidic acid in lipophilic extracts showed some variation among different plant parts and species. Conclusions Lipophilic extracts of Ixora and Greenea species have the potential to be developed as anti-Gram-positive agents, in particular to counter infections of methicillin-resistant S. aureus strains. The chemical profiles showed differences between floristic regions but similarity within the same plant parts.

【 授权许可】

CC BY