Virus-induced gene silencing in the perennial woody Paeonia ostii

PeerJ

article

Lihang Xie¹  Qingyu Zhang¹  Daoyang Sun¹  Weizong Yang¹  Jiayuan Hu¹  Lixin Niu¹  Yanlong Zhang¹ 

[1] College of Landscape Architecture and Arts, Northwest A&F University

关键词: Paeonia ostii;    Virus-induced gene silencing;    Tobacco rattle virus;    Phytoene desaturase;    Green fluorescent protein;   

DOI  :  10.7717/peerj.7001

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Tree peony is a perennial deciduous shrub with great ornamental and medicinal value. A limitation of its current functional genomic research is the lack of effective molecular genetic tools. Here, the first application of a Tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) in the tree peony species Paeonia ostii is presented. Two different approaches, leaf syringe-infiltration and seedling vacuum-infiltration, were utilized for Agrobacterium-mediated inoculation. The vacuum-infiltration was shown to result in a more complete Agrobacterium penetration than syringe-infiltration, and thereby determined as an appropriate inoculation method. The silencing of reporter gene PoPDS encoding phytoene desaturase was achieved in TRV-PoPDS-infected triennial tree peony plantlets, with a typical photobleaching phenotype shown in uppermost newly-sprouted leaves. The endogenous PoPDS transcripts were remarkably down-regulated in VIGS photobleached leaves. Moreover, the green fluorescent protein (GFP) fluorescence was detected in leaves and roots of plants inoculated with TRV-GFP, suggesting the capability of TRV to silence genes in various tissues. Taken together, the data demonstrated that the TRV-based VIGS technique could be adapted for high-throughput functional characterization of genes in tree peony.

【 授权许可】

CC BY