Rosmarinic acid treatment during porcine oocyte maturation attenuates oxidative stress and improves subsequent embryo development in vitro

PeerJ

article

Yan Zhang¹  Jing Guo³  Xiao Wei Nie⁵  Zi Yue Li¹  Yu Meng Wang¹  Shuang Liang⁶  Suo Li¹ 

[1] College of Animal Science and Technology, Jilin Agricultural University;Department of Animal Science, Chungbuk National University;Chongqing Reproductive and Genetics Institute;Chongqing Key Laboratory of Human Embryo Engineering;Department of Reproductive Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine;Department of Animal Science, College of Animal Sciences, Jilin University

关键词: Rosmarinic acid;    Porcine oocyte;    In vitro maturation;    Oxidative stress;   

DOI  :  10.7717/peerj.6930

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundIn vitro maturation (IVM) of oocytes has been widely used in the field of assisted reproductive technology. However, oocytes can be injured by oxidative stress during the process of IVM.MethodsThe present study was designed to evaluate the influences of rosmarinic acid (RA) on the IVM of porcine oocytes and the subsequent development of early-stage embryos as well as its underlying mechanisms. Various concentrations of RA (5 µM, 10 µM, and 25 µM) were treated with porcine oocyte maturation medium during the period of IVM.Results and DiscussionThe results showed that 5 µM RA treatment during the period of porcine oocyte IVM improves blastocyst quality and hatching ability after parthenogenetic activation. Furthermore, the presence of RA during the period of IVM dramatically improved the total number of cells after somatic cell nuclear transfer compared to the number of cells in the control group. Notably, RA treatment during the period of porcine oocyte IVM decreased intracellular reactive oxygen species generation not only in oocytes but also in cumulus cells. Further analysis showed that the intracellular free thiols levels in the oocytes were enhanced by treatment with RA during the period of porcine oocyte IVM compared to the free thiols levels in the control groups. These results indicate that RA improves the developmental competence of porcine oocytes during the IVM period by attenuating oxidative stress.

【 授权许可】

CC BY