Fishing for vaccines against Vibrio cholerae using in silico pan-proteomic reverse vaccinology approach

PeerJ

article

Muhammad I. Rashid¹  Sammia Rehman¹  Amjad Ali¹  Saadia Andleeb¹ 

[1] Department of Industrial Biotechnology, Atta ur Rahman School of Applied Biosciences, National University of Science and Technology

关键词: Vibrio cholerae;    Reverse vaccinology;    Cholera;    Peptide vaccine;    Epitope prediction;   

DOI  :  10.7717/peerj.6223

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundCholera, an acute enteric infection, is a serious health challenge in both the underdeveloped and the developing world. It is caused by Vibrio cholerae after ingestion of fecal contaminated food or water. Cholera outbreaks have recently been observed in regions facing natural calamities (i.e., earthquake in Haiti 2010) or war (i.e., ongoing civil war in Yemen 2016) where healthcare and sanitary setups have been disrupted as a consequence. Whole-cell oral cholera vaccines (OCVs) have been in market but their regimen efficacy has been questioned. A reverse vaccinology (RV) approach has been applied as a successful anti-microbial measure for many infectious diseases.MethodologyWith the aim of finding new protective antigens for vaccine development, the V. cholerae O1 (biovar eltr str. N16961) proteome was computationally screened in a sequential prioritization approach that focused on determining the antigenicity of potential vaccine candidates. Essential, accessible, virulent and immunogenic proteins were selected as potential candidates. The predicted epitopes were filtered for effective binding with MHC alleles and epitopes binding with greater MHC alleles were selected.ResultsIn this study, we report lipoprotein NlpD, outer membrane protein OmpU, accessory colonization factor AcfA, Porin, putative and outer membrane protein OmpW as potential candidates qualifying all the set criteria. These predicted epitopes can offer a potential for development of a reliable peptide or subunit vaccine for V. cholerae.

【 授权许可】

CC BY