Differential mechanisms underlying responses of soil bacterial and fungal communities to nitrogen and phosphorus inputs in a subtropical forest

PeerJ

article

Yong Li¹  Dashuan Tian²  Jinsong Wang²  Shuli Niu²  Jing Tian²  Denglong Ha³  Yuxi Qu³  Guangwei Jing³  Xiaoming Kang¹  Bing Song⁴ 

[1] Beijing Key Laboratory of Wetland Services and Restoration, Institute of Wetland Research;Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research;Jigongshan Natural Reserve;School of Resources and Environmental Engineering, Ludong University

关键词: Nitrogen deposition;    Phosphorus addition;    Microbial diversity;    Community composition;    Amplicon sequencing;    Subtropical forest;    Soil bacteria and fungi;   

DOI  :  10.7717/peerj.7631

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Atmospheric nitrogen (N) deposition and phosphorus (P) addition both can change soil bacterial and fungal community structure with a consequent impact on ecosystem functions. However, which factor plays an important role in regulating responses of bacterial and fungal community to N and P enrichments remains unclear. We conducted a manipulative experiment to simulate N and P inputs (10 g N · m−2 · yr−1 NH4NO3 or 10 g P · m−2 · yr−1 NaH2PO4) and compared their effects on soil bacterial and fungal species richness and community composition. The results showed that the addition of N significantly increased NH4+ and Al3+ by 99.6% and 57.4%, respectively, and consequently led to a decline in soil pH from 4.18 to 3.75 after a 5-year treatment. P addition increased Al3+ and available P by 27.0% and 10-fold, respectively, but had no effect on soil pH. N addition significantly decreased bacterial species richness and Shannon index and resulted in a substantial shift of bacterial community composition, whereas P addition did not. Neither N nor P addition changed fungal species richness, Shannon index, and fungal community composition. A structural equation model showed that the shift in bacterial community composition was related to an increase in soil acid cations. The principal component scores of soil nutrients showed a significantly positive relationship with fungal community composition. Our results suggest that N and P additions affect soil bacterial and fungal communities in different ways in subtropical forest. These findings highlight how the diversity of microbial communities of subtropical forest soil will depend on future scenarios of anthropogenic N deposition and P enrichment, with a particular sensitivity of bacterial community to N addition.

【 授权许可】

CC BY