Effects of shokyo ( Zingiberis Rhizoma ) and kankyo ( Zingiberis Processum Rhizoma ) on prostaglandin E 2 production in lipopolysaccharide-treated mouse macrophage RAW264.7 cells

PeerJ

article

Toshiaki Ara¹  Masanori Koide²  Hiroyuki Kitamura³  Norio Sogawa¹ 

[1] Department of Pharmacology, Matsumoto Dental University;Institute for Oral Science, Matsumoto Dental University;Matsumoto Dental University Hospital

关键词: Kampo medicine;    Kankyo;    Herb;    Shokyo;    Arachidonic acid cascade;    Antiinflammatory effect;    Macrophage;    Prostaglandin E2;    6-shogaol;   

DOI  :  10.7717/peerj.7725

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

We previously reported that shokyo and kankyo, which are water-extracted fractions of ginger, reduced LPS-induced PGE2 production in human gingival fibroblasts. In this study, we examined the effects of these herbs on LPS-treated mouse macrophage RAW264.7 cells. Both shokyo and kankyo reduced LPS-induced PGE2 production in a concentration-dependent manner. Shokyo and kankyo did not inhibit cyclooxygenase (COX) activity, nor did they alter the expression of molecules in the arachidonic acid cascade. In addition, these herbs did not alter NF-κB p65 translocation into nucleus, or phosphorylation of p65 or ERK. These results suggest that shokyo and kankyo inhibit cPLA2 activity. Although 6-shogaol produced similar results to those of shokyo and kankyo, the concentration of 6-shogaol required for the reduction of PGE2 production were higher than those of 6-shogaol in shokyo and kankyo. Therefore, several gingerols and shogaols other than 6-shogaol may play a role in the reduction of LPS-induced PGE2 production. Thus, 6-shogaol, and other gingerols and shogaols inhibit cPLA2 activity and reduce LPS-induced PGE2 production via a different mechanism from traditional anti-inflammatory drugs. Moreover, kampo medicines that contain shokyo or kankyo are considered to be effective for inflammatory diseases.

【 授权许可】

CC BY