Transcriptomic responses of hypothalamus to acute exercise in type 2 diabetic Goto-Kakizaki rats

PeerJ

article

Shuying Fu¹  Yuhuan Meng¹  Shudai Lin¹  Wenlu Zhang¹  Yuting He¹  Lizhen Huang¹  Hongli Du¹ 

[1] School of Biology and Biological Engineering, South China University of Technology

关键词: Acute exercise;    Type 2 diabetes (T2D);    Hypothalamus;    Goto-Kakizaki (GK) rat;    RNA-Seq;    Transcriptome;   

DOI  :  10.7717/peerj.7743

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

The hypothalamus has an integral role in energy homeostasis regulation, and its dysfunctions lead to the development of type 2 diabetes (T2D). Physical activity positively affects the prevention and treatment of T2D. However, there is not much information on the adaptive mechanisms of the hypothalamus. In this study, RNA sequencing was used to determine how acute exercise affects hypothalamic transcriptome from both type 2 diabetic Goto-Kakizaki (GK) and control Wistar rats with or without a single session of running (15 m/min for 60 min). Through pairwise comparisons, we identified 957 differentially expressed genes (DEGs), of which 726, 197, and 98 genes were found between GK and Wistar, exercised GK and GK, and exercised Wistar and Wistar, respectively. The results of Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment revealed that lipid metabolism-related terms and pathways were enriched in GK and exercised GK rats, and nervous system related terms and pathways were enriched in exercised GK and Wistar rats. Furthermore, 45 DEGs were associated with T2D and related phenotypes according to the annotations in the Rat Genome Database. Among these 45 DEGs, several genes (Plin2, Cd36, Lpl, Wfs1, Cck) related to lipid metabolism or the nervous system are associated with the exercise-induced benefits in the hypothalamus of GK rats. Our findings might assist in identifying potential therapeutic targets for T2D prevention and treatment.

【 授权许可】

CC BY