Microbial communities associated with the black morel Morchella sextelata cultivated in greenhouses

PeerJ

article

Gian Maria Niccolò Benucci¹  Reid Longley²  Peng Zhang³  Qi Zhao³  Gregory Bonito¹  Fuqiang Yu³ 

[1] Plant Soil and Microbial Sciences, Michigan State University;Microbiology and Molecular Genetics, Michigan State University;CAS Key Laboratory for East Asia Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences

关键词: Microbiome;    Microbial ecology;    Morchella;    USEARCH;    Mushroom cultivation;    CONSTAX;    Amplicon sequencing;    Pedobacter;   

DOI  :  10.7717/peerj.7744

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Morels (Morchella spp.) are iconic edible mushrooms with a long history of human consumption. Some microbial taxa are hypothesized to be important in triggering the formation of morel primordia and development of fruiting bodies, thus, there is interest in the microbial ecology of these fungi. To identify and compare fungal and prokaryotic communities in soils where Morchella sextelata is cultivated in outdoor greenhouses, ITS and 16S rDNA high throughput amplicon sequencing and microbiome analyses were performed. Pedobacter, Pseudomonas, Stenotrophomonas, and Flavobacterium were found to comprise the core microbiome of M. sextelata ascocarps. These bacterial taxa were also abundant in the soil beneath growing fruiting bodies. A total of 29 bacterial taxa were found to be statistically associated to Morchella fruiting bodies. Bacterial community network analysis revealed high modularity with some 16S rDNA operational taxonomic unit clusters living in specialized fungal niches (e.g., pileus, stipe). Other fungi dominating the soil mycobiome beneath morels included Morchella, Phialophora, and Mortierella. This research informs understanding of microbial indicators and potential facilitators of Morchella ecology and fruiting body production.

【 授权许可】

CC BY