Genome-wide analysis of basic helix–loop–helix superfamily members related to anthocyanin biosynthesis in eggplant ( Solanum melongena L.)

PeerJ

article

Shiyu Tian¹  Lujun Li¹  Min Wei¹  Fengjuan Yang¹ 

[1]College of Horticulture Science and Engineering, Shandong Agricultural University/ State Key Laboratory of Crop Biology

[2]Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, Shandong Agricultural University

[3]Key Laboratory of Biology and Genetic Improvement of Horticultural Crops ,(Huanghuai Region), Ministry of Agriculture

关键词: Solanum melongena;    Expression profile;    bHLH;    Genome-wide;    Anthocyanin biosynthesis;   

DOI  :  10.7717/peerj.7768

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

The basic helix–loop–helix (bHLH) superfamily is considered the second largest transcription factor (TF) family. It plays regulatory roles in the developmental processes of plants and in their defense responses. In recent years, many bHLH superfamily genes have been identified and characterized in herbaceous and woody plants. However, the comprehensive genomic and functional analyses of these genes in eggplant (Solanum melongena L.) have not been reported. In this study, 121 bHLH TFs were identified in the recently released eggplant genome. The phylogeny, gene structure and conserved motifs of the SmbHLH gene were comprehensively studied. Subsequently, the phylogenetic relationship between the bHLH of eggplant and the bHLH of other species was analyzed, and the proteins were classified into 17 subfamilies. Among these protein sequences, 16 subgroups were clustered into the functional clades of Arabidopsis. Two candidate genes (SmbHLH1, SmbHLH117) that may be involved in anthocyanin biosynthesis were screened. The tissue specificity or differential expression of the bHLH genes in different tissues and under various light and temperature conditions suggested the differential regulation of tissue development and metabolism. This study not only provides a solid foundation for the functional dissection of the eggplant bHLH gene family but may also be useful for the future synthesis of anthocyanins in eggplant.

【 授权许可】

CC BY